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Titolo:
Monounsaturated trans fatty acids, elaidic acid and trans-vaccenic acid, metabolism and incorporation in phospholipid molecular species in hepatocytes
Autore:
Woldseth, B; Retterstol, K; Christophersen, BO;
Indirizzi:
Univ Oslo, Rikshosp, Inst Clin Biochem, N-0027 Oslo, Norway Univ Oslo Oslo Norway N-0027 osp, Inst Clin Biochem, N-0027 Oslo, Norway
Titolo Testata:
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
fascicolo: 8, volume: 58, anno: 1998,
pagine: 635 - 645
SICI:
0036-5513(199812)58:8<635:MTFAEA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED RAT-LIVER; PERFORMANCE LIQUID-CHROMATOGRAPHY; PEROXISOMAL RETROCONVERSION; MYOCARDIAL-INFARCTION; CIS-ISOMERS; LONG-CHAIN; CELLS; OCTADECENOATE; MITOCHONDRIA; SEPARATION;
Keywords:
oleic acid; palmitic acid; phosphatidylcholine; phosphatidylethanolamine; rat; stearic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Woldseth, B Univ Oslo, Rikshosp, Inst Clin Biochem, Pilestredet 32, N-0027Oslo, Norway Univ Oslo Pilestredet 32 Oslo Norway N-0027 0027 Oslo, Norway
Citazione:
B. Woldseth et al., "Monounsaturated trans fatty acids, elaidic acid and trans-vaccenic acid, metabolism and incorporation in phospholipid molecular species in hepatocytes", SC J CL INV, 58(8), 1998, pp. 635-645

Abstract

The incorporation of [C-14]elaidic acid (trans18:1(n-9)) in phosphatidylcholine and phosphatidylethanolamine molecular species in isolated rat liver cells has been studied, and the results compared with the incorporation, previously published (B. Woldseth et al. Biochim Biophys Acta 1993;1167:296-302), of [C-14]palmitic acid (16:0) and [C-14]stearic acid (18:0) and with that of [C-14]oleic acid (cis18 : 1(n-9)). The pattern of incorporation in phospholipid molecular species is similar to that of [C-14]stearic acid and different from that of [C-14]palmitic acid. In phosphatidylcholine [C-14]trans18:1-18:2 and [C-14]trans18:1-20:4 were the most abundant species, and in phosphatidylethanolamine [C-14]trans18:1-20:4 was the predominant species. With increasing concentration of [C-14]elaidic acid increasing amounts of[C-14]trans18: 1-[C-14]trans18: 1 were found. The total incorporation in phospholipids was less than that of [C-14]stearic acid, but more than that of [C-14]palmitic acid. The distribution in percent of [C-14]elaidic acid inphospholipid classes was 8.8% in phosphatidylinositol, 1.8% in phosphatidylserine, 59.1% in phosphatidylcholine and 30.3% in phosphatidylethanolaminewith 0.1 mmol l(-1) substrate concentration. More [C-14]elaidic acid than [C-14]palmitic acid or [C-14]stearic acid was oxidized. The incorporation in phospholipids of [C-14]elaidic acid was very different from that of[C-14]oleic acid. The main species with [C-14]oleic acid were 16:0-[C-14]cis18:1 in phosphatidylcholine, and [C-14]cis18:1-20:4 in phosphatidylethanolamine. In some experiments [C-14]18:2(n-6) was incubated together with unlabelledelaidic or unlabelled trans-vaccenic acid (trans18:1(n-7)). In these experiments, more trans18:1-18:2 was formed from elaidic acid than from trans-vaccenic acid, especially in phosphatidylethanolamine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 05:22:17