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Titolo:
Cell-autonomous complementation of mlo resistance using a biolistic transient expression system
Autore:
Shirasu, K; Nielsen, K; Piffanelli, P; Oliver, R; Schulze-Lefert, P;
Indirizzi:
Johnandnes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, Engl John Innes Ctr Plant Sci Res Norwich Norfolk England NR4 7UH orfolk, Engl Carlsberg Lab, Dept Physiol, DK-2500 Copenhagen, Denmark Carlsberg Lab Copenhagen Denmark DK-2500 ol, DK-2500 Copenhagen, Denmark
Titolo Testata:
PLANT JOURNAL
fascicolo: 3, volume: 17, anno: 1999,
pagine: 293 - 299
SICI:
0960-7412(199902)17:3<293:CCOMRU>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
F-SP HORDEI; POWDERY MILDEW; BARLEY; GENES; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Schulze-Lefert, P JohnNorfolk,tr Plant Sci Res, Sainsbury Lab, Colney Lane, Norwich NR4 7UH, John Innes Ctr Plant Sci Res Colney Lane Norwich NorfolkEngland NR4 7UH
Citazione:
K. Shirasu et al., "Cell-autonomous complementation of mlo resistance using a biolistic transient expression system", PLANT J, 17(3), 1999, pp. 293-299

Abstract

The barley gene Mlo encodes a prototype of a novel class of plant proteins. In mlo mutants, absence of the 60 kDa wild-type Mlo protein results in broad-spectrum resistance to the powdery mildew fungus, Erysiphe graminis f, sp. hordei. To directly assess its function, Mlo was transiently expressed with a marker gene encoding a modified green fluorescent protein (GFP) in leaf epidermal cells of mlo resistant barley lines. Fungal inoculation of epidermal cells transfected with wild-type Mlo led to haustorium formation and abundant sporulation. Therefore, expression of the wild-type Mlo gene, inmlo resistant genotypes, is both necessary and sufficient to restore susceptibility to fungal attack. Complementation of mlo resistance alleles was restricted to single host cells, indicating a cell-autonomous function for the wild-type Mlo protein. We discuss our findings with respect to source-sink relationships of plants and biotrophic fungi and the potentially wide-ranging use of the transient complementation assay to analyse host compatibility and defence in response to powdery mildew attack.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:37:09