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Titolo:
Characterization of the translated products of the alternatively spliced luteinizing hormone receptor in the ovine ovary throughout the oestrous cycle
Autore:
Bacich, DJ; Earl, CR; OKeefe, DS; Norman, RJ; Rodgers, RJ;
Indirizzi:
Flinders Univ S Australia, Dept Med, Bedford Pk, SA 5042, Australia Flinders Univ S Australia Bedford Pk SA Australia 5042 SA 5042, Australia Univt,delaide, Queen Elizabeth Hosp, Dept Obstet & Gynaecol, Reprod Med Uni Univ Adelaide Woodville SA Australia 5011 stet & Gynaecol, Reprod Med Uni Straun Res Ctr, Dept Primary Ind, Naracoorte, SA 5271, Australia Straun Res Ctr Naracoorte SA Australia 5271 aracoorte, SA 5271, Australia
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 147, anno: 1999,
pagine: 113 - 124
SICI:
0303-7207(19990125)147:1-2<113:COTTPO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTROPIN-CHORIOGONADOTROPIN RECEPTOR; MESSENGER-RIBONUCLEIC-ACID; LH RECEPTOR; CORPUS-LUTEUM; CELL-TYPES; 3-BETA-HYDROXYSTEROID DEHYDROGENASE; PROGESTERONE PRODUCTION; GONADOTROPIN RECEPTORS; EXTRACELLULAR DOMAIN; GLYCOSYLATION SITES;
Keywords:
luteinizing hormone receptor; ovine; ovary; RNA; splicing; RT-PCR; antibodies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Rodgers, RJ Flinders Univ S Australia, Dept Med, Bedford Pk, SA 5042, Australia Flinders Univ S Australia Bedford Pk SA Australia 5042 tralia
Citazione:
D.J. Bacich et al., "Characterization of the translated products of the alternatively spliced luteinizing hormone receptor in the ovine ovary throughout the oestrous cycle", MOL C ENDOC, 147(1-2), 1999, pp. 113-124

Abstract

The luteinizing hormone receptor (LHR) is alternatively spliced. It is notknown if the alternatively spliced mRNAs are translated in vivo, or indeedif they have any vital role to play. The B splice form has been detected in every species examined, and it encodes a putative protein with a high affinity LH/CG binding domain but no trans-membrane or intra-cellular domains. We raised antisera that recognize the putative protein of the B form, and the closely related G form, and showed that the B form mRNA is translated in the ovine ovary, but not kidney or liver. It localized to the luteal cytosolic and microsomal fractions and the levels declined during regression induced by treatment with prostaglandin F2 alpha. We examined alternative splicing by RNase protection analyses and RT-PCR analyses of healthy pre-ovulatory follicles, atretic or steroidogenically-inactive follicles, and of newly formed, mid-luteal and regressing corpora lutea. There was approximate to 5-fold more B form mRNA than A form. Thus we have evidence that the LHR Bform is translated in vivo, but no evidence that alternative splicing of the LHR mRNA is differentially regulated, throughout the oestrous cycle. (C)1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:28:07