Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Bryostatin-1 and 1 alpha,25-dihydroxyvitamin D-3 synergistically stimulatethe differentiation of NB4 acute promyelocytic leukemia cells
Autore:
Song, XD; Norman, AW;
Indirizzi:
Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 chem, Riverside, CA 92521 USA Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 Sci, Riverside, CA 92521 USA
Titolo Testata:
LEUKEMIA
fascicolo: 2, volume: 13, anno: 1999,
pagine: 275 - 281
SICI:
0887-6924(199902)13:2<275:BA1ADS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ESTER-INDUCED DIFFERENTIATION; PHORBOL ESTER; 1,25-DIHYDROXYVITAMIN D-3; RETINOIC ACID; GENE-EXPRESSION; MACROPHAGE DIFFERENTIATION; MONOCYTIC DIFFERENTIATION; HUMAN-LYMPHOCYTES; ACTIVATION;
Keywords:
bryostatin-1; 1,25-dihydroxyvitamin D-3; alkaline phosphatase; NB4 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Norman, AW Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 side, CA 92521 USA
Citazione:
X.D. Song e A.W. Norman, "Bryostatin-1 and 1 alpha,25-dihydroxyvitamin D-3 synergistically stimulatethe differentiation of NB4 acute promyelocytic leukemia cells", LEUKEMIA, 13(2), 1999, pp. 275-281

Abstract

One of the objectives of treatment for patients with acute promyelocytic leukemia (APL) is to induce tumor cell differentiation and block cell proliferation. Acute promyelocytic leukemia cells (NB4) responded to the combination treatment of 1 alpha,25-dihydroxyvitamin D-3 [1 alpha,25(OH)(2)D-3] plus phorbol 12-myristate 13-acetate (PMA) and differentiated into monocyte/macrophage-like cells, as well as expressed strong alkaline phosphatase (ALP)activities. Since PMA has limited clinical application due to its tumor-promoting effect, another protein kinase C activator, bryostatin-1, was currently tested for its interaction with 1 alpha,25(OH)(2)D-3 to induce NB4 cell differentiation and block cell proliferation. Bryostatin-1 alone, but not1 alpha,25(OH)(2)D-3 alone, significantly inhibited cell proliferation andinduced NB4 cell differentiation into monocyte/macrophages; however neither bryostatin-1 nor 1 alpha,25(OH)(2)D-3 alone induced ALP expression. Like PMA, bryostatin-1 synergistically interacted with 1 alpha,25(OH)(2)D-3 to stimulate ALP expression 30-fold over the control (P < 0.001) and further promote appearance of monocyte/macrophage-like cells. The ALP stimulation wasboth time- and dose-dependent. Thus, we demonstrate for the first time that the combination of bryostatin-1 and 1 alpha,25(OH)(2)D-3 strongly affect NB4 cell differentiation and proliferation. Therefore, this proposed combination treatment may be an alternatively potential therapeutic regimen for APL patients and assay of ALP may be a more sensitive and facile way to monitor the possible remission of APL patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:07:43