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Titolo:
CURRENT TRENDS IN MOLECULAR RECOGNITION AND BIOSEPARATION
Autore:
JONES C; PATEL A; GRIFFIN S; MARTIN J; YOUNG P; ODONNELL K; SILVERMAN C; PORTER T; CHAIKEN I;
Indirizzi:
SMITHKLINE BEECHAM PHARMACEUT,DEPT MOLEC IMMUNOL,709 SWEDELAND RD KING OF PRUSSIA PA 19406 SMITHKLINE BEECHAM PHARMACEUT,DEPT MOLEC IMMUNOL KING OF PRUSSIA PA 19406 SMITHKLINE BEECHAM PHARMACEUT,DEPT PROT BIOCHEM KING OF PRUSSIA PA 19406
Titolo Testata:
Journal of chromatography
fascicolo: 1, volume: 707, anno: 1995,
pagine: 3 - 22
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-PLASMON RESONANCE; SINGLE-STEP PURIFICATION; QUANTITATIVE AFFINITY CHROMATOGRAPHY; BIOSPECIFIC INTERACTION ANALYSIS; HIV-1 REVERSE-TRANSCRIPTASE; ESCHERICHIA-COLI; HUMAN RENIN; PLASMINOGEN-ACTIVATOR; RAPID PURIFICATION; BINDING CONSTANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
96
Recensione:
Indirizzi per estratti:
Citazione:
C. Jones et al., "CURRENT TRENDS IN MOLECULAR RECOGNITION AND BIOSEPARATION", Journal of chromatography, 707(1), 1995, pp. 3-22

Abstract

Molecular recognition guides the selective interaction of macromolecules with each other in essentially all biological processes. Perhaps the most impactful use of biomolecular recognition in separation science has been in affinity chromatography. The results of the last 26 years, since Cuatrecases, Wilchek and Anfinsen first reported the purification of staphylococcal nuclease, have validated the power of biomolecular specificity for purification. This power has stimulated an explosion of solid-phase ligand designs and affinity chromatographic applications. An ongoing case in point is the purification of recombinant proteins, which has been aided by engineering the proteins to contain Affinity-Tag sequences, such as hexa-histidine for metal-chelate separation and epitope sequence for separation by an immobilized monoclonal antibody. Tag technology can be adapted for plate assays and other solid-phase techniques. The advance of affinity chromatography also has stimulated immobilized ligand-based methods to characterize macromolecularrecognition, including both chromatographic and optical biosensor methods. And, new methods such as phage display and other diversity library approaches continue to emerge to identify new recognition moleculesof potential use as affinity ligands. Overall, it is tantalizing to envision a continued evolution of new affinity technologies which use the selectivity built into biomolecular recognition as a vehicle for purification, analysis, screening and other applications in separation sciences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:26:15