Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Direct selection for mutators in Escherichia coli
Autore:
Miller, JH; Suthar, A; Tai, J; Yeung, A; Truong, C; Stewart, JL;
Indirizzi:
UnivSAalif Los Angeles, Dept Microbiol & Mol Genet, Los Angeles, CA 90095 U Univ Calif Los Angeles Los Angeles CA USA 90095 , Los Angeles, CA 90095 U Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 5, volume: 181, anno: 1999,
pagine: 1576 - 1584
SICI:
0021-9193(199903)181:5<1576:DSFMIE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MISMATCH REPAIR DEFICIENCY; HIGH MUTATION-RATES; MICROSATELLITE INSTABILITY; COLORECTAL-CANCER; CELL-LINES; SPONTANEOUS MUTAGENESIS; FRAMESHIFT MUTATIONS; GENE; DNA; STRAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, JH UnivSAalif Los Angeles, Dept Microbiol & Mol Genet, Los Angeles, CA 90095 U Univ Calif Los Angeles Los Angeles CA USA 90095 es, CA 90095 U
Citazione:
J.H. Miller et al., "Direct selection for mutators in Escherichia coli", J BACT, 181(5), 1999, pp. 1576-1584

Abstract

We have constructed strains that allow a direct selection for mutators of Escherichia coli on a single plate medium. The plate selection is based on using two different markers whose reversion is enhanced by a given mutator,Plates containing limiting amounts of each respective nutrient allow the growth of ghost colonies or microcolonies that give rise to full-size colonies only if a reversion event occurs. Because two successive mutational events are required, mutator cells are favored to generate full-size colonies. Reversion of a third marker allows direct visualization of the mutator phenotype by the large number of blue papillae in the full-size colonies. We also describe plate selections involving three successive nutrient markers followed by a fourth papillation step. Different frameshift or base substitution mutations are used to select for mismatch-repair-defective strains (mutHLS and uvrD). We can detect and monitor mutator cells arising spontaneously, at frequencies lower than 10(-5) in the population. Also, we can measurea mutator cascade, in which one type of mutator (mutT) generates a second mutator (mutHLS) that then allows stepwise frameshift mutations. We discussthe relevance of mutators arising on a single medium as a result of cells overcoming successive growth barriers to the development and progression ofcancerous tumors, some of which are mutator cell lines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 19:28:18