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Titolo:
INVESTIGATION INTO CELL-GROWTH ON COLLAGEN CHONDROITIN-6-SULFATE GELS- THE EFFECT OF CROSS-LINKING AGENTS AND DIAMINES/
Autore:
OSBORNE CS; REID WH; GRANT MH;
Indirizzi:
UNIV STRATHCLYDE,WOLFSON CTR,BIOENGN UNIT,106 ROTTENROW GLASGOW G4 0NW LANARK SCOTLAND GLASGOW ROYAL INFIRM,BURNS UNIT GLASGOW G4 0NW LANARK SCOTLAND
Titolo Testata:
Journal of materials science. Materials in medicine
fascicolo: 4, volume: 8, anno: 1997,
pagine: 179 - 184
SICI:
0957-4530(1997)8:4<179:IICOCC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPIDERMAL-KERATINOCYTES; COLLAGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C.S. Osborne et al., "INVESTIGATION INTO CELL-GROWTH ON COLLAGEN CHONDROITIN-6-SULFATE GELS- THE EFFECT OF CROSS-LINKING AGENTS AND DIAMINES/", Journal of materials science. Materials in medicine, 8(4), 1997, pp. 179-184

Abstract

Artificial skin substitutes based on cultured autologous keratinocytes need to have sufficient strength and ease of handling to be utilizedsuccessfully by surgeons in the clinic. This may be achieved by crosslinking the collagen substratum on which the cells are cultured, whichin this case is a collagen gel. Increased strength must be attained without detrimental effect on cell growth. The influence of potential crosslinking agents including the glycosaminoglycan, chondroitin-6-sulphate (ChGSO(4)), the water soluble carbodiimide crosslinking agents 1-ethyl-3-(3-diaminopropyl) carbodiimide (EDAC), and 1,1-carbonyldiimidazole (CDl), and the polyamines putrescine, spermine and diaminohexane,on cell growth rate has been investigated. Incorporation of 20% ChGSO(4) into collagen gels caused an approximately 16% increase in keratinocyte growth, but had no significant effect on that of dermal fibroblasts. Pre-formed collagen gels (+/- ChGSO(4)) were treated with the carbodiimides. This crosslinking treatment markedly inhibited fibroblast growth (EDAC 45% inhibition, CDl 70%), without affecting that of keratinocytes. Pre-formed collagen gels (+/- ChGSO(4) and carbodiimide) were treated with 0.1 M, 0.5 M or 1.0 M polyamine. Spermine inhibited thegrowth rate of both cell types at all concentrations tested, whereas putrescine and diaminohexane had little effect. The mechanical strength of these crosslinked gels is currently being assessed to determine the optimum composition in terms of cell growth and biocompatibility, and strength.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 16:36:16