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Titolo:
Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity
Autore:
Trachtman, H; Futterweit, S; Koss, E; Bogart, M; Mermelstein, A;
Indirizzi:
Schneiderteinldrens Hosp, Div Nephrol, Long Isl Jewish Med Ctr, Albert Eins Schneider Childrens Hosp New Hyde Park NY USA 11040 Med Ctr, Albert Eins
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 3, volume: 3, anno: 1999,
pagine: 323 - 327
SICI:
1107-3756(199903)3:3<323:GORMCO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN; MECHANISMS; GLYCATION; PRODUCTS; KIDNEY;
Keywords:
mesangium; inducible nitric oxide synthase; metal-catalyzed oxidation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Trachtman, H Schneiderteinldrens Hosp, Div Nephrol, Long Isl Jewish Med Ctr, Albert Eins Schneider Childrens Hosp 269-01 76th Ave,Long Isl Campus NewHyde Park NY USA 11040
Citazione:
H. Trachtman et al., "Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity", INT J MOL M, 3(3), 1999, pp. 323-327

Abstract

Biochemical modification of extracellular matrix (ECM) proteins can alter the function in overlying cells. We tested the hypothesis that metal-catalyzed oxidation of native ECM and individual matrix proteins modulates the activity of inducible nitric oxide synthase (iNOS) in cultured rat mesangial cells (RMC). Oxidized modification of native ECM resulted in a 32% increasein iNOS activity (P<0.01) without influencing the response to supplementalL-arginine or to the addition of the iNOS inhibitor, L-NAME. Immunoblot analysis indicated that enhanced iNOS activity was not associated with a parallel rise in the cytosolic content of iNOS. Synthesis of type IV collagen was unaffected by growth of RMC on oxidized native ECM. Oxidation of three normal constituents of the mesangial matrix-type IV collagen, laminin, and fibronectin-also stimulated iNOS activity in overlying RMC by 18-32% (P<0.05). Growth of RMC on oxidized type I collagen or Vitrogel had no effect on NO production. We conclude that oxidized modification of the mesangial matrix promotes increased iNOS activity and NO production by mesangial cells. Further work is required to determine whether this response limits glomerularinjury or promotes damage to the mesangium in oxygen free radical-mediateddiseases such as chronic renal failure, atherosclerosis and diabetes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 17:00:40