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Titolo:
HETEROGENEOUS TOPOGRAPHIC AND CELLULAR-DISTRIBUTION OF HUNTINGTON EXPRESSION IN THE NORMAL HUMAN NEOSTRIATUM
Autore:
FERRANTE RJ; GUTEKUNST CA; PERSICHETTI F; MCNEIL SM; KOWALL NW; GUSELLA JF; MACDONALD ME; BEAL MF; HERSCH SM;
Indirizzi:
BEDFORD VA MED CTR,GERIATR RES EDUC CLIN CTR,UNIT 182B,200 SPRINGS RDBEDFORD MA 01730 BOSTON UNIV,SCH MED,DEPT NEUROL BOSTON MA 02118 EMORY UNIV,SCH MED,DEPT NEUROL ATLANTA GA 30322 MASSACHUSETTS GEN HOSP,MOL NEUROGENET UNIT CHARLESTOWN MA 02129 MASSACHUSETTS GEN HOSP,NEUROCHEM LAB,NEUROL SERV BOSTON MA 02114 HARVARD UNIV,SCH MED BOSTON MA 02114
Titolo Testata:
The Journal of neuroscience
fascicolo: 9, volume: 17, anno: 1997,
pagine: 3052 - 3063
SICI:
0270-6474(1997)17:9<3052:HTACOH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-BINDING PROTEIN; DISEASE GENE HOMOLOG; STRIATAL NEURONS; BASAL GANGLIA; WIDESPREAD EXPRESSION; NADPH-DIAPHORASE; SPINY NEURONS; SUBSTANCE-P; BRAIN; RAT;
Keywords:
HUNTINGTONS DISEASE; HUNTINGTON; NEOSTRIATUM; IMMUNOFLUORESCENCE; NADPH-DIAPHORASE; NITRIC OXIDE SYNTHASE; CALBINDIN D28K;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Ferrante et al., "HETEROGENEOUS TOPOGRAPHIC AND CELLULAR-DISTRIBUTION OF HUNTINGTON EXPRESSION IN THE NORMAL HUMAN NEOSTRIATUM", The Journal of neuroscience, 17(9), 1997, pp. 3052-3063

Abstract

A striking heterogeneous distribution of topographic and cellular huntingtin immunoreactivity was observed within the human neostriatum using three distinct huntingtin antibodies. Patchy areas of low huntingtin immunoreactivity were present in both the caudate nucleus and putamen, surrounded by an intervening area of greater immunoreactivity. Comparison of huntingtin immunoreactivity with contiguous serial sections stained for enkephalin and calbindin D28k immunoreactivities showed that the topographic heterogeneity of huntingtin immunostaining corresponded to the patch (striosome) and matrix compartments within the striatum. Huntingtin immunoreactivity was confined primarily to neurons andneuropil within the matrix compartment, whereas little or no neuronalor neuropil huntingtin immunostaining was observed within the patch compartment. There was marked variability in the intensity of huntingtin immunolabel among medium-sized striatal neurons, whereas a majority of large striatal neurons were only faintly positive or without any immunoreactivity. Combined techniques for NADPH-diaphorase enzyme histochemistry and huntingtin immunocytochemistry, as well as double immunofluorescence for either nitric oxide synthase or calbindin D28k in comparison with huntingtin expression, revealed a striking correspondence between calbindin D28k and huntingtin immunoreactivities, with little or no colocalization between NADPH-diaphorase or nitric oxide synthaseneurons and huntingtin expression. These observations suggest that the selective vulnerability of spiny striatal neurons and the matrix compartment observed in Huntington's disease is associated with higher levels of huntingtin expression, whereas the relative resistance of large and medium-sized aspiry neurons and the patch compartments to degeneration is associated with low levels of huntingtin expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:16:47