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Titolo:
Increased nerve growth factor mRNA in lateral calf skin biopsies from diabetic patients
Autore:
Diemel, LT; Cai, F; Anand, P; Warner, G; Kopelman, PG; Fernyhough, P; Tomlinson, DR;
Indirizzi:
Univlanddon Queen Mary & Westfield Coll, Dept Pharmacol, London E1 4NS, Eng Univ London Queen Mary & Westfield Coll London England E1 4NS 1 4NS, Eng
Titolo Testata:
DIABETIC MEDICINE
fascicolo: 2, volume: 16, anno: 1999,
pagine: 113 - 118
SICI:
0742-3071(199902)16:2<113:INGFMI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETROGRADE AXONAL-TRANSPORT; MESSENGER-RNA EXPRESSION; FACTOR GENE; NEUROTROPHIC FACTORS; TOPICAL CAPSAICIN; FACTOR RECEPTORS; SENSORY NEURONS; CHAIN-REACTION; SUBSTANCE-P; SPINAL-CORD;
Keywords:
diabetes mellitus; human; neuropathy; nerve growth factor; neurotrophin; cRT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Tomlinson, DR Univeranchester, Div Neurosci, Room 1-124 Stopford Bldg,Oxford Rd, Manchest Univ Manchester Room 1-124 Stopford Bldg,Oxford Rd Manchester Lancs England M13 9PT
Citazione:
L.T. Diemel et al., "Increased nerve growth factor mRNA in lateral calf skin biopsies from diabetic patients", DIABET MED, 16(2), 1999, pp. 113-118

Abstract

Aims This study set out to establish a novel procedure for the measurementof human nerve growth factor (NGF) messenger ribonucleic acid (mRNA) and to use this method to measure NGF expression in skin biopsies from control subjects and from patients with early neuropathies. NGF mRNA levels were related to functional measures of the competence of NGF-responsive nerves. Methods mRNA levels were measured by competitive reverse transcription with polymerase chain reaction amplification (cRT-PCR). Functional correlates of this observation were assessed by indices of thermal sensitivity - mediated by C-fibres, whose phenotype is regulated by NGF. Results NGF mRNA was increased in skin biopsies from 19 diabetic patients (5.12+/-3.88 (sD)) compared with samples from eight controls (1.57+/-0.95; P=0.001). Diabetic patients showed significantly (P<0.001) diminished detection of cool and warm stimuli compared to age matched control group (n=24),but there were no differences in detection of heat as pain, or correlationwith NGF mRNA levels. Conclusions These findings suggest abnormally increased expression of NGF in diabetic neuropathy, which may represent a compensatory mechanism for impaired phenotype in NGF-responsive neurones.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:30:41