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Titolo:
Determination of biogenic amines in fermented beverages and vinegars by pre-column derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl) and reversed-phase LC
Autore:
Kirschbaum, J; Meier, A; Bruckner, H;
Indirizzi:
Univ Giessen, Dept Food Sci, Inst Nutr Sci, D-35390 Giessen, Germany Univ Giessen Giessen Germany D-35390 Nutr Sci, D-35390 Giessen, Germany
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 3-4, volume: 49, anno: 1999,
pagine: 117 - 124
SICI:
0009-5893(199902)49:3-4<117:DOBAIF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHIC METHOD; PRECOLUMN DERIVATIZATION; FOODS; EXTRACTION; WINES;
Keywords:
column liquid chromatography; pre-column derivatization; para-nitrobenzyloxycarbonyl chloride; biogenic amines; fermented beverages;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Bruckner, H Univermanyen, Dept Food Sci, Inst Nutr Sci, Sudanlage 6, D-35390 Giessen, G Univ Giessen Sudanlage 6 Giessen Germany D-35390 0 Giessen, G
Citazione:
J. Kirschbaum et al., "Determination of biogenic amines in fermented beverages and vinegars by pre-column derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl) and reversed-phase LC", CHROMATOGR, 49(3-4), 1999, pp. 117-124

Abstract

The reagent p-nitrobenzyloxycarbonyl chloride (PNZ-CI) was used for pre-column derivatization of biogenic amines (BAs) at ambient temperature followed by reversed-phase, liquid-chromatographic separation of the derivatives. Optimized derivatization of samples was achieved within 10 min in berate buffer by adding PNZ-CI in acetonitrile (MeCN). Excess reagent was scavenged by subsequent addition of glycine in water. For LC a Superspher(R) RP-18e column and gradient elution using a ternary gradient system containing sodium acetate buffer (pH 6.1), sodium acetate buffer (pH 4.3) and MeCN, were used. The PNZ-derivatives were quan tified by their UV-absorption at 265 nm. Detection limits of BAs were approximately 62-1000 mu g L-l (injected amounts: 53-850 pg) at a signal-to-noise ratio of 3:1. The coefficients of determination were 0.9906-0.9992. Diaminohexane was used as internal standard. Recoveries of BAs ranged from 78-93 % depending on the food matrix. This method was applied to the quantitative determination of 2-phenylethylamine, tryptamine, serotonin, putrescine, histamine, cadaverine, tyramine, spermidine, and spermine, in beer, wine, vinegars, and lactic fermented cabbage juice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:47:01