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Titolo:
Down-regulation of oxytocin-induced cyclooxygenase-2 and prostaglandin F synthase expression by interferon-tau in bovine endometrial cells
Autore:
Xiao, CW; Murphy, BD; Sirois, J; Goff, AK;
Indirizzi:
Univ Montreal, Fac Med Vet, Ctr Rech Reprod Anim, St Hyacinthe, PQ J2S 7C6, Univ Montreal St Hyacinthe PQ Canada J2S 7C6 m, St Hyacinthe, PQ J2S 7C6,
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 3, volume: 60, anno: 1999,
pagine: 656 - 663
SICI:
0006-3363(199903)60:3<656:DOOCAP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CHORIONIC-GONADOTROPIN; OVINE ENDOMETRIUM; EARLY-PREGNANCY; MESSENGER-RNA; OVARIECTOMIZED EWES; EPITHELIAL-CELLS; INDUCED RELEASE; IN-VIVO; TROPHOBLAST INTERFERONS; RECEPTOR CONCENTRATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Goff, AK Univinthe,eal, Fac Med Vet, Ctr Rech Reprod Anim, 3200 Rue Sicotte, St Hyac Univ Montreal 3200 Rue Sicotte St Hyacinthe PQ Canada J2S 7C6 yac
Citazione:
C.W. Xiao et al., "Down-regulation of oxytocin-induced cyclooxygenase-2 and prostaglandin F synthase expression by interferon-tau in bovine endometrial cells", BIOL REPROD, 60(3), 1999, pp. 656-663

Abstract

Oxytocin (OT) is responsible for the episodic release of luteolytic prostaglandin (PC) F-2 alpha from the uterus in ruminants. The attenuation of OT-stimulated uterine PGF(2 alpha) secretion by interferon-tau (IFN-tau) is essential for prevention of luteolysis during pregnancy in cows. To better understand the mechanisms involved, the effect of recombinant bovine IFN-tau (rbIFN-tau) on OT-induced PG production and cyclooxygenase-2 (COX-2) and PCF synthase (PGFS) expression in cultured endometrial epithelial cells was investigated. Cells were obtained from cows at Days 1-3 of the estrous cycleand cultured to confluence in RPMI medium supplemented with 5% steroid-free fetal calf serum. The cells were then incubated in the presence or absence of either 100 ng/ml OT or OT + 100 ng/ml rbIFN-tau for 3, 6, 12, and 24 h. OT significantly increased PGF(2 alpha) and PGE(2) secretion at all time points (p < 0.01), while rbIFN-tau inhibited the OT-induced PG production and reduced OT receptor binding in a time-dependent manner. OT increased thesteady-state level of COX-2 mRNA, measured by Northern blot, which was maximal at 3 h (9-fold increase) and then decreased with time (p < 0.01). OT also caused an increase in COX-2 protein, which peaked at 12 h (Il-fold increase), as measured by Western blot. Addition of rbIFN-tau suppressed the induction of COX-2 mRNA (89%, p < 0.01) and COX-2 protein (50%, p < 0.01) by OT. OT also increased PGFS mRNA, and this stimulation was attenuated by rbIFN-tau (p < 0.01). To ensure that the decrease in COX-2 was not solely due to down-regulation of the OT receptor, cells were stimulated with a phorbolester (phorbol 12-myristate 13-acetate; PMA) in the presence and absence of rbIFN-tau. The results showed that rbIFN-tau also decreased PMA-stimulated PC production and COX-2 protein. It can be concluded that rbIFN-tau inhibition of OT-stimulated PC production is due to down-regulation of OT receptor, COX-2, and PGFS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 22:55:53