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Titolo:
Canine preprorelaxin: Nucleic acid sequence and localization within the canine placenta
Autore:
Klonisch, T; Hombach-Klonisch, S; Froehlich, C; Kauffold, J; Steger, K; Steinetz, BG; Fischer, B;
Indirizzi:
Univnyalle Wittenberg, Dept Anat & Cell Biol, Fac Med, D-06097 Halle, Germa Univ Halle Wittenberg Halle Germany D-06097 ac Med, D-06097 Halle, Germa Univ7010pzig, Fac Med Vet, Large Anim Clin Theriogenol & Ambulance Serv, D- Univ Leipzig Leipzig Germany D-7010 lin Theriogenol & Ambulance Serv, D- NYU, Nelson Inst Environm Med, Med Ctr, Tuxedo Park, NY 10987 USA NYU Tuxedo Park NY USA 10987 ronm Med, Med Ctr, Tuxedo Park, NY 10987 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 3, volume: 60, anno: 1999,
pagine: 551 - 557
SICI:
0006-3363(199903)60:3<551:CPNASA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN RELAXIN; GENE-EXPRESSION; EARLY-PREGNANCY; EQUINE RELAXIN; CLEAVAGE SITES; SERUM RELAXIN; RAT; PROGESTERONE; SYNCYTIOTROPHOBLAST; TROPHOBLAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Klonisch, T Univ-06097 Wittenberg, Dept Anat & Cell Biol, Fac Med, Grosse Steiner 52, D Univ Halle Wittenberg Grosse Steiner 52 Halle Germany D-06097
Citazione:
T. Klonisch et al., "Canine preprorelaxin: Nucleic acid sequence and localization within the canine placenta", BIOL REPROD, 60(3), 1999, pp. 551-557

Abstract

Employing uteroplacental tissue at Day 35 of gestation, we determined the nucleic acid sequence of canine preprorelaxin using reverse transcription- and rapid amplification of cDNA ends-polymerase chain reaction. Canine preprorelaxin cDNA consisted of 534 base pairs encoding a protein of 177 amino acids with a signal peptide of 25 amino acids (aa), a B domain of 35 aa, a C domain of 93 aa, and an A domain of 24 aa. The putative receptor binding region in the N'-terminal part of the canine relaxin B domain GRDYVR contained two substitutions from the classical motif (E-->D and L-->Y). Canine preprorelaxin shared highest homology with porcine and equine preprorelaxin. Northern analysis revealed a l-kilobase transcript present in total RNA of canine uteroplacental tissue but not of kidney tissue. Uteroplacental tissue from two bitches each at Days 30 and 35 of gestation were studied by in situ hybridization to localize relaxin mRNA. Immunohistochemistry for relaxin, cytokeratin, vimentin, and von Willebrand factor was performed on uteroplacental tissue at Day 30 of gestation. The basal cell layer at the core ofthe chorionic villi was devoid of relaxin mRNA and immunoreactive relaxin or vimentin but was immunopositive for cytokeratin and identified as cytotrophoblast cells. The cell layer surrounding the chorionic villi displayed specific hybridization signals for relaxin mRNA and immunoreactivity for relaxin and cytokeratin but not for vimentin, and was identified as syncytiotrophoblast. Those areas of the chorioallantoic tissue with most intense relaxin immunoreactivity were highly vascularized as demonstrated by immunoreactive von Willebrand factor expressed on vascular endothelium. The uterine glands and non-placental uterine areas of the canine zonary girdle placenta were devoid of relaxin mRNA and relaxin. We conclude that the syncytiotrophoblast is the source of relaxin in the canine placenta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 15:57:02