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Titolo:
Blood ethanol levels and adenylyl cyclase activity in lymphocytes of alcoholic patients
Autore:
Pauly, T; Dahmen, N; Szegedi, A; Wetzel, H; Bol, GF; Ferdinand, K; Hiemke, C;
Indirizzi:
Univ Mainz, Dept Psychiat, D-55131 Mainz, Germany Univ Mainz Mainz Germany D-55131 , Dept Psychiat, D-55131 Mainz, Germany Univ Dusseldorf, Inst Physiol Chem 2, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 2, D-4000 Dusseldorf, Germany
Titolo Testata:
BIOLOGICAL PSYCHIATRY
fascicolo: 4, volume: 45, anno: 1999,
pagine: 489 - 493
SICI:
0006-3223(19990215)45:4<489:BELAAC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; G-PROTEINS; RECEPTORS; CAMP; CELLS; ASSAY; CHLORMETHIAZOLE; NUCLEOTIDES; ACTIVATION; SEPARATION;
Keywords:
adenylyl cyclase; cyclic adenosine monophosphate; ethanol; lymphocytes; alcoholism; second messenger;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Hiemke, C Univ Mainz, Dept Psychiat, Untere Zahlbacher Str 8, D-55131 Mainz, Germany Univ Mainz Untere Zahlbacher Str 8 Mainz Germany D-55131 ermany
Citazione:
T. Pauly et al., "Blood ethanol levels and adenylyl cyclase activity in lymphocytes of alcoholic patients", BIOL PSYCHI, 45(4), 1999, pp. 489-493

Abstract

Background: The adenylyl cyclase (AC) signal transduction pathway is a target of acute and chronic ethanol actions. This study examined whether AC activity in lymphocyte membranes of male alcoholic patients correlated with blood concentrations of ethanol. Methods: Patients (n = 13; mean age: 40 +/- 8 years) were studied on the day of admission (day 0) and 2 days Inter under detoxification. Moreover 13 age-matched male healthy controls (mean age 40 +/- 9 years) were included. Lymphocyte membranes were prepared by differential centrifugation whereby blood ethanol was washed out, As a measure of AC activity the formation of cyclic adenosine monophosphate (cAMP) from adenosine triphosphate was determined without (basal activity) and with stimulation of the second messenger system by the guanosine triphosphate (GTP) analogue GTP gamma S (20 mu mol/L) via the G-protein or by forskolin (100 mu mol/L) acting directly on the AC enzyme,Results: On day 0, when ethanol blood concentrations were 38-100 mmol/L, we found a significant negative correlation between ethanol blood levels andstimulated AC activities. On day 2, the negative con-elation with blood ethanol levels of day 0 had disappearedConclusions: The consumption of ethanol affects the AC system in lymphocytes of alcohol-dependent patients by a persistent effect on the cAMP formingenzyme. (C) 1999 Society of Biological Psychiatry.

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Documento generato il 23/01/20 alle ore 12:36:00