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Titolo:
Predominance of the acylation route in the metabolic processing of exogenous sphingosine in neural and extraneural cells in culture
Autore:
Riboni, L; Bassi, R; Prinetti, A; Viani, P; Tettamanti, G;
Indirizzi:
UnivITA,an, Dept Med Chem & Biochem, Study Ctr Func Biochem Brain Lipids, L Univ Milan Segrate Milan Italy I-20090 y Ctr Func Biochem Brain Lipids, L
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 338, anno: 1999,
parte:, 1
pagine: 147 - 151
SICI:
0264-6021(19990215)338:<147:POTARI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE CELLS; SPHINGOLIPID METABOLISM; DIFFERENTIATION; CERAMIDE; SPHINGANINE; GANGLIOSIDE; INHIBITION; SPHINGOMYELIN; BIOSYNTHESIS; INDUCTION;
Keywords:
N-acylation; ceramide; sphingosine metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Tettamanti, G UnivITA,an, Dept Med Chem & Biochem, Study Ctr Func Biochem Brain Lipids, L Univ Milan Via Fratelli Cervi 93 Segrate Milan Italy I-20090
Citazione:
L. Riboni et al., "Predominance of the acylation route in the metabolic processing of exogenous sphingosine in neural and extraneural cells in culture", BIOCHEM J, 338, 1999, pp. 147-151

Abstract

The metabolic fate of exogenous [H-3]sphingosine was investigated in five types of cultured cells: primary cultures of neurons and astrocytes, murineand human neuroblastoma cells and human skin fibroblasts. After administration of 40 nM [3-H-3]sphingosine into a cell-conditioned medium containing fetal calf serum, all cell types rapidly and efficiently incorporated the long-chain base in a time-dependent fashion. In all cases, after a 120 min pulse, the amount of radioactivity taken up was in the range of the endogenous sphingosine content. However, unchanged [H-3]sphingosine represented only a very minor portion of the label incorporated into cells throughout the pulse period (10-120 min), indicating rapid and efficient sphingosine metabolism in these cells. Most of the [H-3]sphingosine taken up was metabolically processed, either by degradation (assessed as (H2O)-H-3 release into theculture medium) or by N-acylation (mainly to radioactive ceramide, sphingomyelin, neutral glycolipids and gangliosides). [H-3]Sphingosine 1-phosphateaccounted for less than 2 % of the total radioactivity incorporated in allcases. Throughout the pulse period and in all cell types, H-3-labelled organic metabolites largely prevailed over (H2O)-H-3, indicating that N-acylation is the major metabolic fate of sphingosine in these cells under apparently physiological conditions. These results are consistent with the notion that sphingosine has a rapid turnover in the cells studied, and indicate that regulation of the basal level of this bioactive molecule occurs mainly through N-acylation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:44:56