Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Thrombopoietin-induced conformational change in p53 lies downstream of thep44/p42 mitogen activated protein kinase cascade in the human growth factor-dependent cell line M07e
Autore:
Ritchie, A; Braun, SE; He, J; Broxmeyer, HE;
Indirizzi:
Indiana Univ, Sch Med, Dept Microbiol Immunol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 mmunol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Med Hematol Oncol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 Oncol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ol Ctr, Indianapolis, IN 46202 USA Walther Canc Inst, Indianapolis, IN 46208 USA Walther Canc Inst Indianapolis IN USA 46208 t, Indianapolis, IN 46208 USA
Titolo Testata:
ONCOGENE
fascicolo: 7, volume: 18, anno: 1999,
pagine: 1465 - 1477
SICI:
0950-9232(19990218)18:7<1465:TCCIPL>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; FACTOR RECEPTOR SUPERFAMILY; TUMOR-SUPPRESSOR PROTEIN; MPL CYTOPLASMIC DOMAIN; C-KIT LIGAND; TYROSINE-PHOSPHORYLATION; HEMATOPOIETIC-CELLS; MEGAKARYOCYTIC DIFFERENTIATION; SHC PHOSPHORYLATION; SIGNALING MOLECULES;
Keywords:
MAPK; p53 conformation; thrombopoietin; apoptosis; M07e;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Broxmeyer, HE Indiana Univ, Sch Med, Dept Microbiol Immunol, Indianapolis,IN 46202 USA Indiana Univ Indianapolis IN USA 46202 apolis, IN 46202 USA
Citazione:
A. Ritchie et al., "Thrombopoietin-induced conformational change in p53 lies downstream of thep44/p42 mitogen activated protein kinase cascade in the human growth factor-dependent cell line M07e", ONCOGENE, 18(7), 1999, pp. 1465-1477

Abstract

Thrombopoietin is a cytokine with potent megakaryocytopoietic and thrombopoietic activities in who. Wild-type p53 is a conformationally flexible, anti-oncogenic transcription factor that plays a principal role in mediating growth factor withdrawal-induced apoptosis in factor-dependent hematopoieticcells. We recently reported that Tpo induces a conformational change in and functional inactivation of p53, coincident with its anti-apoptotic effects, in the human factor-dependent cell line M07e. In an effort to identify potential signaling cascades through which Tpo illicits these effects on p53, we report here that treating M07e cells with MAPK kinase inhibitor PD98059 dramatically suppressed Tpo-induced conformational change in p53 as well as Tpo-enhanced viability in M07e cells in a p53-dependent manner. Furthermore, the expression of constitutively active Raf1 in M07e cells induced conformational change in p53 independent of Tpo stimulation. Inhibition of theJAK/STAT pathway revealed that JAK/STAT signaling plays an insignificant role in conformational modulation of p53 and apoptosis suppression. Inhibition of phosphatidylinositol-3 kinase did not have a significant effect on p53 conformation but did have a weak but significant effect on Tpo-enhanced viability. Cytokine-induced activation of the MAPK pathway and the subsequent functional neutralization of p53, may be an event by which apoptosis is commonly suppressed in hematopoiesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:01:31