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Titolo:
Synthesis and enzymatic processing of oligodeoxynucleotides containing tandem base damage
Autore:
Bourdat, AG; Gasparutto, D; Cadet, J;
Indirizzi:
CEAl,renoble, Dept Rech Fondamentale Mat Condensee, Serv Chim Inorgan & Bio CEA Grenoble Grenoble France 9 le Mat Condensee, Serv Chim Inorgan & Bio
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 4, volume: 27, anno: 1999,
pagine: 1015 - 1024
SICI:
0305-1048(19990215)27:4<1015:SAEPOO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
8-OXOGUANINE DNA GLYCOSYLASE; ESCHERICHIA-COLI; SUBSTRATE-SPECIFICITY; FPG PROTEIN; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; 2-DEOXYRIBOSYLFORMAMIDE RESIDUE; CHEMICAL SYNTHESIS; OGG1 GENE; CLONING; RADIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Cadet, J CEAl,renoble, Dept Rech Fondamentale Mat Condensee, Serv Chim Inorgan & Bio CEA Grenoble Grenoble France 9 ndensee, Serv Chim Inorgan & Bio
Citazione:
A.G. Bourdat et al., "Synthesis and enzymatic processing of oligodeoxynucleotides containing tandem base damage", NUCL ACID R, 27(4), 1999, pp. 1015-1024

Abstract

Several studies have shown that ionizing radiation generates a wide spectrum of lesions to DNA including base modifications, abasic sites, strand breaks, crosslinks and tandem base damage. One example of tandem base damage induced by OH radical in X-irradiated DNA oligomers is N-(2-deoxy-beta-D-erythropentofuranosyl)-formylamine/8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), In order to investigate the biological significance of such a tandem lesion, both 8-oxo-7,8-dihydroguanine and formylamine were introduced into synthetic oligonucleotides at vicinal positions using the solid phase phosphoramidite method. For this purpose, a new convenient method of synthesis of 8-oxodGuo was developed. The purity and integrity of the modified synthetic DNA fragments were assessed using different complementary techniques including HPLC, polyacrylamide gel electrophoresis, electrospray and MALDI-TOFmass spectrometry. The piperidine test applied to the double modified base-containing oligonucleotides revealed the high alkaline lability of formylamine in DNA, In addition, various enzymatic experiments aimed at determining biochemical features of such multiply damaged sites were carried out using the synthetic substrates, The processing of the vicinal lesions by nuclease Fl, snake venom phosphodiesterase, calf spleen phosphodiesterase and repair enzymes including Escherichia coli endonuclease (endo) III and Fapy-glycosylase was studied and is reported.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:19:21