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Titolo:
Yeast forward and reverse 'n'-hybrid systems
Autore:
Vidal, M; Legrain, P;
Indirizzi:
MGH Canc Ctr, Charlestown, MA 02129 USA MGH Canc Ctr Charlestown MA USA 02129 Canc Ctr, Charlestown, MA 02129 USA Inst Pasteur, Lab Metab ARNs, CNRS, URA 1300, F-75724 Paris 15, France Inst Pasteur Paris France 15 s, CNRS, URA 1300, F-75724 Paris 15, France Hubrigenics, F-75724 Paris 15, France Hubrigenics Paris France 15Hubrigenics, F-75724 Paris 15, France
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 4, volume: 27, anno: 1999,
pagine: 919 - 929
SICI:
0305-1048(19990215)27:4<919:YFAR'S>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-PROTEIN INTERACTIONS; PAPILLOMAVIRUS TYPE-16 E1; INTERACTIONS IN-VIVO; MAP KINASE CASCADE; 2-HYBRID SYSTEM; GENETIC SELECTION; RETINOBLASTOMA PROTEIN; MOLECULAR-CLONING; BINDING-PROTEIN; 3-HYBRID SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
93
Recensione:
Indirizzi per estratti:
Indirizzo: Vidal, M MGH Canc Ctr, Charlestown, MA 02129 USA MGH Canc Ctr CharlestownMA USA 02129 Charlestown, MA 02129 USA
Citazione:
M. Vidal e P. Legrain, "Yeast forward and reverse 'n'-hybrid systems", NUCL ACID R, 27(4), 1999, pp. 919-929

Abstract

Since its original description almost 10 years ago, the yeast two-hybrid system has been used extensively to identify protein-protein interactions from many different organisms, Simultaneously, a number of 'variations on a theme' based on the original concept have been described, In one set of variations, systems were developed to detect other macromolecular interactions:DNA-protein (one-hybrid), RNA-protein (RNA-based three-hybrid) and small molecule-protein interactions (ligand-based three-hybrid), These different versions are collectively referred to here as "n-hybrid systems', In anotherset of variations, the original configuration of the two-hybrid fusion proteins was modified to expand the range of possible protein-protein interactions that could be analyzed. For example, systems were developed to detect trimeric interactions, ligand-receptor interactions or interactions that require particular post-translational modifications. Finally, the original concept was turned upside down and 'reverse n-hybrid systems' were developed to identify mutations, peptides or small molecules that dissociate macromolecular interactions, These reagents can be used to validate, in the relevant biological systems, the potential interactions identified with the 'forward n-hybrid systems', The powerful genetic selections of the forward and reverse n-hybrid systems are proving useful in proteomic projects aimed at generating macromolecular interaction maps.

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Documento generato il 04/12/20 alle ore 22:40:52