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Titolo:
Expression of neurotrophins in hippocampal interneurons immunoreactive forthe neuropeptides somatostatin, neuropeptide-Y, vasoactive intestinal polypeptide and cholecystokinin
Autore:
Pascual, M; Acsady, L; Rocamora, N; Freund, TF; Soriano, E;
Indirizzi:
Univpaincelona, Fac Biol, Dept Anim & Plant Cell Biol, E-08028 Barcelona, S Univ Barcelona Barcelona Spain E-08028 t Cell Biol, E-08028 Barcelona, S Hungarian Acad Sci, Inst Expt Med, H-1450 Budapest, Hungary Hungarian AcadSci Budapest Hungary H-1450 Med, H-1450 Budapest, Hungary
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 89, anno: 1999,
pagine: 1089 - 1101
SICI:
0306-4522(199904)89:4<1089:EONIHI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; SEPTAL CHOLINERGIC NEURONS; FACTOR MESSENGER-RNA; RAT HIPPOCAMPUS; GABAERGIC NEURONS; LIMBIC SEIZURES; INHIBITORY INTERNEURONS; SYNAPTIC CONNECTIONS; DIFFERENT SUBSETS; PYRAMIDAL CELLS;
Keywords:
hippocampus; neurotrophin expression; GABAergic interneurons; neuropeptides; septohippocampal afferents;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Pascual, M Univ8Barcelona, Fac Biol, Dept Anim & Plant Cell Biol, Diagonal645, E-0802 Univ Barcelona Diagonal 645 Barcelona Spain E-08028 45, E-0802
Citazione:
M. Pascual et al., "Expression of neurotrophins in hippocampal interneurons immunoreactive forthe neuropeptides somatostatin, neuropeptide-Y, vasoactive intestinal polypeptide and cholecystokinin", NEUROSCIENC, 89(4), 1999, pp. 1089-1101

Abstract

Using a double detection method, which combines in situ hybridization for the detection of neurotrophin messenger RNA with immunocytochemistry against the neuropeptides somatostatin, neuropeptide Y, vasoactive intestinal polypeptide and cholecystokinin, we have analysed the expression of the neurotrophins; nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3, in distinct populations of neuropeptide-immunoreactive hippocampalinterneurons. Nerve growth factor messenger RNA expression was found in subsets of the four subpopulations of neuropeptide-immunoreactive interneurons. The highest degree of co-localization was observed in the neuropeptide-Y-positive cells (up to 70%) and in somatostatin-immunoreactive cells (48%). Only small subsets of cholecystokinin- and vasoactive intestinal polypeptide-positive neurons (21% and 10%, respectively) displayed nerve growth factor hybridization signals. In contrast, expression of neurotrophin-3 messenger RNA was exclusively observed in 26% of neuropeptide-Y-immunoreactive cells. Brain-derived neurotrophic factor hybridization signals were never detected in the neuropeptide-positive hippocampal interneurons. Morphological analysis of neuropeptide-immunoreactive interneurons that express or lack nerve growth factor messenger RNA revealed that most perisomatic inhibitory neurons, such as large vasoactive intestinal polypeptide/ cholecystokinin-immunoreactive cells, showed positive nerve growth factor hybridization signals. In addition, some somatostatin/neuropeptide-Y-immunoreactive interneurons, which are responsible for dendritic inhibition of principal hippocampalneurons, expressed nerve growth factor messenger RNA. In contrast. interneurons specialized to innervate other GABAergic cells, such as small vasoactive intestinal polypeptide-positive cells, lacked nerve growth factor expression. All these data indicate that expression of neurotrophins is differentially regulated in functionally distinct classes of hippocampal interneurons immunoreactive for neuropeptides. We also analysed whether neuropeptide-immunoreactive interneurons expressing neurotrophins were targets of the GABAergic septohippocampal pathway. We used a triple detection method, combining anterograde tracing of this connection, with in situ hybridization for the detection of neurotrophin mRNA, and immunocytochemistry against neuropeptides. Our data showed that the four populations of hippocampal interneurons studied (somatostatin, neuropeptide-Y, vasoactive intestinal polypeptide and cholecystokinin) received GABAergic afferents from the septum. However, no preference for neuropeptide-immunoreactive cells expressing neurotrophins wasobserved, compared to neuropeptide-positive neurons lacking neurotrophin expression. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:08:15