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Titolo:
Mutagenesis of the Neisseria gonorrhoeae porin reduces invasion in epithelial cells and enhances phagocyte responsiveness
Autore:
Bauer, FJ; Rudel, T; Stein, M; Meyer, TF;
Indirizzi:
Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany Max Planck Inst Biol Tubingen Germany D-72076 D-72076 Tubingen, Germany Max Planck Inst Infekt Biol, D-10117 Berlin, Germany Max Planck Inst Infekt Biol Berlin Germany D-10117 10117 Berlin, Germany
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 3, volume: 31, anno: 1999,
pagine: 903 - 913
SICI:
0950-382X(199902)31:3<903:MOTNGP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL TRANSFORMATION COMPETENCE; ESCHERICHIA-COLI; PROTEIN-I; PATHOGENIC NEISSERIA; SHUTTLE MUTAGENESIS; BACTERIAL ENTRY; OMPF PORIN; NEUTROPHILS; GONOCOCCI; CONSTRUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, TF Maxmanynck Inst Biol, Infekt Biol Abt, Spemannstr 34, D-72076 Tubingen, Ger Max Planck Inst Biol Spemannstr 34 Tubingen Germany D-72076 Ger
Citazione:
F.J. Bauer et al., "Mutagenesis of the Neisseria gonorrhoeae porin reduces invasion in epithelial cells and enhances phagocyte responsiveness", MOL MICROB, 31(3), 1999, pp. 903-913

Abstract

Porin (PorB), the major outer membrane protein of Neisseria gonorrhoeae, has been implicated in pathogenesis previously. However, the fact that porindeletion mutants are not viable has complicated investigations. Here, we describe a method of manipulating the porin gene site-specifically. N. gonorrhoeae MS11, which harbours the porB(1B) (P.1B) porin allele, was used to generate mutants carrying deletions in the surface loops 1 and 5. An 11-amino-acid deletion in loop 1 impaired Opa(50)-dependent invasion into human Chang epithelial cells, whereas loop 5 deletion exhibited no apparent phenotype. In a second approach, the complete gonococcal porB(1B) was replaced by the porB(Nla) gene of Neisseria lactamica, Such mutants were unable to induce efficient uptake by epithelial cells but induced an enhanced respiratoryresponse in HL60 phagocytic cells. The increased respiratory burst was accompanied by an enhanced phagocytic uptake of the mutant compared with the wild-type strain, Our data extend previous evidence for multiple central functions of PorB in the infection process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:08:51