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Titolo:
Involvement of calyculin A-sensitive phosphatase(s) in the differentiationof Trypanosoma cruzi trypomastigotes to amastigotes
Autore:
Grellier, P; Blum, J; Santana, J; Bylen, E; Mouray, E; Sinou, V; Teixeira, ARL; Schrevel, J;
Indirizzi:
Museumtaire,Hist Nat, EP CNRS 1790 Biol & Evolut Parasites, Lab Biol Parasi Museum Natl Hist Nat Paris France 05 & Evolut Parasites, Lab Biol Parasi Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ DurhamNC USA 27710 d Ctr, Dept Cell Biol, Durham, NC 27710 USA Univsilia,lia, Lab Multidisciplinar Pesquisa Doenca Chagas, BR-70919970 Bra Univ Brasilia Brasilia DF Brazil BR-70919970 BCca Chagas, BR-70919970 Bra
Titolo Testata:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
fascicolo: 2, volume: 98, anno: 1999,
pagine: 239 - 252
SICI:
0166-6851(19990125)98:2<239:IOCAPI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; MAMMALIAN-CELLS; EPIMASTIGOTE FORMS; OKADAIC ACID; HIGH-DENSITY; LOW PH; INVASION; PHOSPHORYLATION; NEURAMINIDASE; CYTOSKELETON;
Keywords:
trypanosome; Chagas disease; differentiation; phosphatase; calyculin A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Grellier, P Museumtaire,Hist Nat, EP CNRS 1790 Biol & Evolut Parasites, Lab Biol Parasi Museum Natl Hist Nat 61 Rue Buffon Paris France 05 iol Parasi
Citazione:
P. Grellier et al., "Involvement of calyculin A-sensitive phosphatase(s) in the differentiationof Trypanosoma cruzi trypomastigotes to amastigotes", MOL BIOCH P, 98(2), 1999, pp. 239-252

Abstract

Differentiation of the non-dividing trypomastigote form of Trypanosoma cruzi, the causative agent of Chagas disease, to the dividing amastigote form normally occurs in cytoplasm of infected cells. Here we show that calyculinA, a potent inhibitor of protein phosphatases 1 and 2A, induces at pH 7.5 extracellular transformation of long slender trypomastigotes to round amastigote-like forms which acquire characteristic features observed after the normal differentiation process: repositioning and structural changes of the kinetoplast, release of surface neuraminidase, and expression of amastigote-specific epitopes. Calyculin A inhibits parasite phosphatases and changes in the phosphorylation of specific proteins occur during the transformationprocess. As an exposure of trypomastigotes to calyculin A concentrations as low as 1 nM and for only 1-2 h is sufficient to induce transformation, the inhibition of calyculin A-sensitive phosphatase(s) appears to play a major role in initiating the trypomastigote differentiation. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:38:03