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Titolo:
Differential effect of N-ethyl maleimide on Delta 6-desaturase activity inhuman fetal liver toward fatty acids of the n-6 and n-3 series
Autore:
Rodriguez, A; Sarda, P; Boulot, P; Leger, CL; Descomps, B;
Indirizzi:
Faclier,Montpellier, Inst Biol, Lab Biol & Biochim Lipides, F-34060 Montpel Fac Med Montpellier Montpellier France F-34060 Lipides, F-34060 Montpel CHU Montpellier, Hop Arnaud Villeneuve, Serv Pediat 2, Montpellier, FranceCHU Montpellier Montpellier France , Serv Pediat 2, Montpellier, France CHUrancepellier, Hop Arnaud Villeneuve, Serv Gynecol Obstet, Montpellier, F CHU Montpellier Montpellier France Serv Gynecol Obstet, Montpellier, F
Titolo Testata:
LIPIDS
fascicolo: 1, volume: 34, anno: 1999,
pagine: 23 - 30
SICI:
0024-4201(199901)34:1<23:DEONMO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSOMES; RAT; INTERCONVERSION; CHROMATOGRAPHY; PHOSPHOLIPIDS; DESATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Descomps, B FacAMed Montpellier, Inst Biol, Lab Biol & Biochim Lipides, Blvd Henri IV,E Fac Med Montpellier Blvd Henri IV,EA DRED 2033 Montpellier France F-34060
Citazione:
A. Rodriguez et al., "Differential effect of N-ethyl maleimide on Delta 6-desaturase activity inhuman fetal liver toward fatty acids of the n-6 and n-3 series", LIPIDS, 34(1), 1999, pp. 23-30

Abstract

The effect of N-ethyl-maleimide (NEM) on Delta 5- and Delta 6-desaturase activities and the incorporation of substrates and products into different microsomal lipid classes and phospholipid (PL) subclasses were studied in human fetal liver microsomes, obtained after legally approved therapeutic abortion. Desaturase activities were measured by a radiochemical method using reversed-phase high-performance liquid chromatography (HPLC). After nonphospholipid (NPL) and PL separation on silica cartridges, the radioactivity indifferent lipids of the NPL group was assessed by two-dimensional thin-layer chromatography, and their fatty acid (FA) composition by gas-liquid chromatography. The PL subclasses were separated, and the distribution of radioactivity between products and substrates was determined in PL subclasses. NEM inhibited the Delta 5- and Delta 6-desaturase activities in the n-6 series of FA but not the Delta 6-desaturase activity in the n-3 series, which suggests the existence of two distinct Delta 6-desaturases, one for the n-6 series and another for the n-3 series. Whether NEM was present or absent, most of the radioactivity was recovered in the free FA form (about 80%). Thedesaturation products, obtained in the presence or absence of NEM, were preferentially incorporated into FL, suggesting a channeling of the newly synthesized FA toward microsomal FL. The comparison of the distribution of substrates and products incorporated into the different PL classes showed thatmost of the labeled FA were incorporated into phosphatidylcholine and to alesser degree into phosphatidylethanolamine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:36:50