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Titolo:
Molecular arrangement in two-dimensional streptavidin crystals
Autore:
Wang, SW; Robertson, CR; Gast, AP;
Indirizzi:
Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ept Chem Engn, Stanford, CA 94305 USA
Titolo Testata:
LANGMUIR
fascicolo: 4, volume: 15, anno: 1999,
pagine: 1541 - 1548
SICI:
0743-7463(19990216)15:4<1541:MAITSC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-DIMENSIONAL PROTEIN CRYSTALLIZATION; LIPID LAYERS; SURFACE; CRYSTALLOGRAPHY; RECOGNITION; MICROSCOPY; DOMAINS; AVIDIN; GROWTH; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Gast, AP Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Engn, Stanford, CA 94305 USA
Citazione:
S.W. Wang et al., "Molecular arrangement in two-dimensional streptavidin crystals", LANGMUIR, 15(4), 1999, pp. 1541-1548

Abstract

We studied the molecular arrangement of two-dimensional streptavidin crystals at the air-water interface over a range of pH values. We quantified thevarying amounts of coexisting P1, P2, and C222 crystals in the different morphologies observed at pH 4.5-6.5. Chiral, needlelike crystals at pH 4.5 consist of P1 crystals with frequent line defects. Larger chiral domains near pH 5 are essentially all P1 coexisting with a small amount of P2, whereasat slightly higher pH values (near pH 5.5), II-shaped domains contain 4 times as much P1 coexisting with a P2/C222 mixture. Morphologies intermediateto these shapes exhibit intermediate compositions. Between pH similar to 6-7, crystals all display a characteristic dendritic-X morphology, but arrangement at the molecular level is quite different compared with lower pH values. Crystals are mostly P2 in symmetry near pH 6, but at pH 7 and above, crystals have C222 symmetry. Coexistence of P2 and C222 crystals occurs at intermediate pH values. We determined the orientation and arrangement of streptavidin molecules in P1, P2, and C222 crystals relative to the directionsexhibiting faster growth. The direction of faster growth in P1 crystals includes both interactions between biotin-free subunits and interactions between biotin-bound subunits. In the P2 arrangement, growth in the direction of intermolecular biotin-free subunits is preferred, whereas growth is faster along the biotin-bound direction of C222 crystals. We developed a model of the molecular arrangement for the observed solid-phase coexistence in these crystals.

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Documento generato il 24/10/20 alle ore 11:25:14