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Titolo:
BEN/SC1/DM-GRASP expression during neuromuscular development: a cell adhesion molecule regulated by innervation
Autore:
Fournier-Thibault, C; Pourquie, O; Rouaud, T; Le Douarin, NM;
Indirizzi:
Fac Sci & Tech, CNRS, F-44322 Nantes 03, France Fac Sci & Tech Nantes France 03 & Tech, CNRS, F-44322 Nantes 03, France Coll France, F-94736 Nogent Sur Marne, France Coll France Nogent Sur Marne France F-94736 736 Nogent Sur Marne, France CNRS, Inst Embryol Cellulaire & Mol, F-94736 Nogent Sur Marne, France CNRS Nogent Sur Marne France F-94736 l, F-94736 Nogent Sur Marne, France Inst45,ol Dev Marseille, Lab Genet & Physiol Dev, CNRS, Unite Mixte Rech 65 Inst Biol Dev Marseille Marseille France 09 v, CNRS, Unite Mixte Rech 65
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 19, anno: 1999,
pagine: 1382 - 1392
SICI:
0270-6474(19990215)19:4<1382:BEDNDA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED MUSCLE-CELLS; CHICK-EMBRYO; SKELETAL-MUSCLE; IMMUNOGLOBULIN SUPERFAMILY; ACETYLCHOLINE-RECEPTORS; NEURITE EXTENSION; POLYSIALIC ACID; DM-GRASP; CADHERIN EXPRESSION; SYNAPTIC PLASTICITY;
Keywords:
adhesion molecule; neuromuscular development; synaptic sites; nerve control; chick embryo; cell recognition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Fournier-Thibault, C UnivtParis 06, CNRS, Unite Mixte Rech 7622, Batiment C,7 Eme Etage,9 Quai S Univ Paris 06 Batiment C,7 Eme Etage,9 Quai St Bernard Paris France 05
Citazione:
C. Fournier-Thibault et al., "BEN/SC1/DM-GRASP expression during neuromuscular development: a cell adhesion molecule regulated by innervation", J NEUROSC, 19(4), 1999, pp. 1382-1392

Abstract

BEN/SC1/DM-GRASP is a cell adhesion molecule belonging to the lg superfamily that is transiently expressed during avian embryogenesis in a variety ofcell types, including the motoneurons of the spinal cord. We have investigated the pattern of BEN expression during neuromuscular development of the chick. We show that both motoneurons and their target myoblasts express BENduring early embryonic development and that the protein becomes restrictedat neuromuscular contacts as soon as postsynaptic acetylcholine receptor clusters are observed in muscle fibers. Muscle cells grown in vitro express and maintain BEN expression even when they fuse and give rise to mature myotubes. When embryos are deprived of innervation by neural tube ablation, BEN expression is observed in muscle fibers, whereas, in control, the proteinis already restricted at neuromuscular synaptic sites. These results demonstrate that all myogenic cells intrinsically express BEN and maintain the protein in the absence of innervation. Conversely, when neurons are added to myogenic cultures, BEN is rapidly downregulated in muscle cells, demonstrating that innervation controls the restricted pattern of BEN expression seen in innervated muscles. after nerve section in postnatal muscles, BEN protein becomes again widely spread over muscle fibers. When denervated muscles are allowed to be reinnervated, the protein is reexpressed in regenerating motor axons, and reinnervation of synaptic sites leads to the concentration of BEN at neuromuscular junctions. Our results suggest that BEN cell adhesion molecule acts both in the formation of neuromuscular contacts during development and in the events leadingto muscle reinnervation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:04:28