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Titolo:
Subcellular localization of chromogranins, calcium channels, amine carriers, and proteins of the exocytotic machinery in bovine splenic nerve
Autore:
Leitner, B; Lovisetti-Scamihorn, P; Heilmann, J; Striessnig, J; Blakely, RD; Eiden, LE; Winkler, H;
Indirizzi:
Innsbruck Univ, Dept Pharmacol, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 macol, A-6020 Innsbruck, Austria Innsbruck Univ, Dept Biochem Pharmacol, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 macol, A-6020 Innsbruck, Austria Vanderbilt Univ, Med Ctr, Ctr Mol Neurosci, Nashville, TN USA Vanderbilt Univ Nashville TN USA tr, Ctr Mol Neurosci, Nashville, TN USA NIMH,2Mol Neurosci Sect, Lab Cellular & Mol Regulat, NIH, Bethesda, MD 2089 NIMH Bethesda MD USA 20892 Cellular & Mol Regulat, NIH, Bethesda, MD 2089
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 3, volume: 72, anno: 1999,
pagine: 1110 - 1116
SICI:
0022-3042(199903)72:3<1110:SLOCCC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSE CORE VESICLES; VESICULAR MONOAMINE TRANSPORTER; SECRETOGRANIN-II SECRETONEURIN; ADRENAL CHROMAFFIN GRANULES; SMALL SYNAPTIC VESICLES; TOXIN LIGHT-CHAIN; TETANUS TOXIN; IMMUNOLOGICAL CHARACTERIZATION; PROHORMONE CONVERTASES; REGULATED EXOCYTOSIS;
Keywords:
NESP55; synaptophysin; VAMP; VMAT2 carrier; syntaxin; SNAP-25;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Winkler, H InnsbruckaUniv, Dept Pharmacol, Peter Mayr Str 1A, A-6020 Innsbruck, Austri Innsbruck Univ Peter Mayr Str 1A Innsbruck Austria A-6020 stri
Citazione:
B. Leitner et al., "Subcellular localization of chromogranins, calcium channels, amine carriers, and proteins of the exocytotic machinery in bovine splenic nerve", J NEUROCHEM, 72(3), 1999, pp. 1110-1116

Abstract

Subcellular fractionation of bovine splenic nerves, which consist mainly of sympathetic nerve fibers, has been useful for characterizing cellular organelles en route to the terminal. In the present study we have characterized the subcellular distribution of both secretory and membrane proteins. A newly discovered chromogranin-like protein, NESP55, was found in large dense-core vesicles. The endogenous processing of NESP55 was comparable to that of chromogranins but more limited than that of secretogranin II and chromogranin B. For membrane proteins three major types of distribution were found. The amine carrier VMAT2 was confined to large dense-core vesicles. VAMP or synaptobrevin was present both in large dense-core vesicles and in lighter vesicles, whereas SNAP-25, syntaxin, and two types (N and L) of Ca2+ channels were found in a special population of lighter vesicles but were not present in large dense-core vesicles or at the most in very low concentrations. The plasma membrane norepinephrine transporter was apparently present ina separate type of vesicle, but this requires further study. These resultsfurther characterize vesicles en route to the terminal and establish for the first time that peptides involved in exocytosis (syntaxin, SNAP-25, and N- and L-type Ca2+ channels) are apparently transported to the terminal in a special type of vesicle. The exclusive presence of the amine carrier in large dense-core vesicles indicates that the formation of small dense-core vesicles in the terminals requires a reuse of membrane components of large dense-core vesicles.

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Documento generato il 05/04/20 alle ore 09:45:51