Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evaluation of methodologies including immunofluorescent assay (IFA) and the polymerase chain reaction (PCR) for detection of human pathogenic microsporidia in water
Autore:
Dowd, SE; Gerba, CP; Kamper, M; Pepper, IL;
Indirizzi:
Univ Arizona, Dept Microbiol & Immunol, Tucson, AZ 85721 USA Univ ArizonaTucson AZ USA 85721 icrobiol & Immunol, Tucson, AZ 85721 USA Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA Univ Arizona Tucson AZ USA 85721 ter & Environm Sci, Tucson, AZ 85721 USA
Titolo Testata:
JOURNAL OF MICROBIOLOGICAL METHODS
fascicolo: 1, volume: 35, anno: 1999,
pagine: 43 - 52
SICI:
0167-7012(199902)35:1<43:EOMIIA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROCYTOZOON-BIENEUSI; MICROBIAL DNA; INFECTIONS; EXTRACTION; AIDS; IDENTIFICATION; EUKARYOTES; DIAGNOSIS; PARASITE; PATIENT;
Keywords:
microsporidia; PCR; waterborne pathogen; IFA; water; detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Dowd, SE 429 Shantz Bldg 38, Tucson, AZ 85721 USA 429 Shantz Bldg 38 Tucson AZ USA 85721 38, Tucson, AZ 85721 USA
Citazione:
S.E. Dowd et al., "Evaluation of methodologies including immunofluorescent assay (IFA) and the polymerase chain reaction (PCR) for detection of human pathogenic microsporidia in water", J MICROB M, 35(1), 1999, pp. 43-52

Abstract

Microsporidia is a term used to describe a group of emerging protozoan pathogens whose environmental occurrence has only recently been documented dueto lack of detection methodologies. This study evaluates and describes current methods for detection of microsporidia in water. Standard methods, forthe collection and processing of large volumes of water to detect protozoa, showed only a 4.8% recovery, of microsporidia spores, from 100 I volumes of tap. Immunofluorescent assay (IFA) analysis was assessed using two different antibodies specific for human pathogenic microsporidia. Results indicated that the use of IFA for routine screening of water for microsporidia was not an acceptable approach. The antibodies tested for the IFA resulted infalse positives and false negatives and did not react with Enterocytozoon bieneusi, which is an important human pathogenic microsporidia. Finally, the small sizes of the human pathogenic microsporidia prevent confirmation and species determination by light microscopic methods. Two methods for isolating microsporidia DNA from water for use in polymerase chain reaction (PCR) amplification of microsporidia target sequences were assessed. Both of these DNA isolation methods when combined with the PCR showed the ability to detect less than ten spores in purified water concentrates. Thus, this study represents the first documentation and evaluation of current methods for the detection of human pathogenic microsporidia in water. (C) 1999 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:50:47