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Titolo:
Distinct "assisted" and "spontaneous" mechanisms for the insertion of polytopic chlorophyll-binding proteins into the thylakoid membrane
Autore:
Kim, SJ; Jansson, S; Hoffman, NE; Robinson, C; Mant, A;
Indirizzi:
Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England Umea Univ, Dept Plant Physiol, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 , Dept Plant Physiol, S-90187 Umea, Sweden Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA CarnegieInst Washington Stanford CA USA 94305 ol, Stanford, CA 94305 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 8, volume: 274, anno: 1999,
pagine: 4715 - 4721
SICI:
0021-9258(19990219)274:8<4715:D"A"MF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-RECOGNITION PARTICLE; ESCHERICHIA-COLI; CHLOROPLAST HOMOLOG; PRECURSOR PROTEIN; INNER MEMBRANE; PHOTOSYSTEM-II; INTEGRATION; TRANSPORT; POLYPEPTIDE; MACHINERY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, C Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL nds, England
Citazione:
S.J. Kim et al., "Distinct "assisted" and "spontaneous" mechanisms for the insertion of polytopic chlorophyll-binding proteins into the thylakoid membrane", J BIOL CHEM, 274(8), 1999, pp. 4715-4721

Abstract

The biogenesis of several bacterial polytopic membrane proteins has been shown to require signal recognition particle (SRP) and protein transport machinery, and one such protein, the major light-harvesting chlorophyll-binding protein (LHCP) exhibits these requirements in chloroplasts. In this report we have used in vitro insertion assays to analyze four additional membersof the chlorophyll-alb-binding protein family. We show that two members, Lhca1 and Lhcb5, display an absolute requirement for stroma, nucleoside triphosphates, and protein transport apparatus, indicating an "assisted" pathway that probably resembles that of LHCP. Two other members, however, namely an early light-inducible protein 2 (Elip2) and photosystem II subunit S (PsbS), can insert efficiently in the complete absence of SRP, SecA activity, nucleoside triphosphates, or a functional Sec system. The data suggest a possibly spontaneous insertion mechanism that, to date, has been characterized only for simple single-span proteins. Of the membrane proteins whose insertion into thylakoids has been analyzed, five have now been shown to insertby a SRP/Sec-independent mechanism, suggesting that this is a mainstream form of targeting pathway. We also show that PsbS and Elip2 molecules are capable of following either "unassisted" or assisted pathways, and we discussthe implications for the mechanism and role of SRP in chloroplasts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 21:04:58