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Titolo:
Effect of cholecystokinin blockade on the recovery of alterations induced by acute pancreatitis in glycoconjugates of rat zymogen granules
Autore:
De Dios, I; Garcia-Montero, AC; Orfao, A; Manso, MA;
Indirizzi:
Univ Salamanca, Dept Physiol & Pharmacol, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 & Pharmacol, Salamanca 37007, Spain Univ Salamanca, Serv Flow Cytometry, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 w Cytometry, Salamanca 37007, Spain
Titolo Testata:
GLYCOCONJUGATE JOURNAL
fascicolo: 9, volume: 15, anno: 1998,
pagine: 923 - 928
SICI:
0282-0080(199809)15:9<923:EOCBOT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROCORTISONE; SECRETION; CERULEIN; CCK; ANTAGONIST; L-364,718; PROTEIN; INVITRO; MOIETY; JUICE;
Keywords:
acute pancreatitis; zymogen granule glycoconjugates; cholecystokinin-receptor antagonist; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: De Dios, I Univ Salamanca, Dept Physiol & Pharmacol, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 , Salamanca 37007, Spain
Citazione:
I. De Dios et al., "Effect of cholecystokinin blockade on the recovery of alterations induced by acute pancreatitis in glycoconjugates of rat zymogen granules", GLYCOCON J, 15(9), 1998, pp. 923-928

Abstract

Lectin-binding studies have been performed on rat zymogen granules to investigate alterations in the carbohydrate membrane composition that occur in acute pancreatitis induced by caerulein. The influence of treatment with hydrocortisone for seven days before inducing pancreatitis was also studied. Lectin labeling on zymogen granules was also analyzed seven days after inducing pancreatitis in rats that had previously received a hydrocortisone treatment. During this period L 364,718 (0.1 mg/kg)-specific cholecystokinin (CCK) receptor antagonist-was administered daily to some of the rats, and notreatment was applied to others. Using fluorescein-labelled T. purpureus (TP) lectin, a significant decrease in the amount of L-fucose in the granulemembrane was observed in rats with caerulein-induced pancreatitis. This effect was directly caused by the pancreatitis and was not influenced by previous hydrocortisone treatment. Seven days later, the density of TP receptors in the granule membrane was similar to the controls both in L-364,718-treated and untreated rats. Therefore, we suggest that endogenous CCK is not an essential factor in the recovery of L-fucose containing glycoconjugates the granule membrane after pancreatitis. Acute pancreatitis did not alter the expression of wheat germ agglutinin (WGA) receptors in the zymogen granule membrane. WGA specifically binds N-acetyl glucosamine and sialic acids, L364,718 administered for seven days after inducing pancreatitis significantly reduced WGA binding, untreated rats showed a normal zymogen granule membrane. Therefore, the blockade of CCK-induced alterations in membrane glycoconjugates enriched in N-acetyl glucosamine and sialic acid of newly formedgranules after pancreatitis, a finding that could explain the delay in theregression of the disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:40:51