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Titolo:
Molecular characterization of a carbapenem-hydrolyzing beta-lactamase fromChryseobacterium (Flavobacterium) indologenes
Autore:
Bellais, S; Leotard, S; Poirel, L; Naas, T; Nordmann, P;
Indirizzi:
Hoparis,tre, Serv Bacteriol Virol, Fac Med Paris Sud, Assistance Publ Hop P Hop Bicetre Le Kremlin Bicetre France F-94275 Sud, Assistance Publ Hop P Hopedntoine Beclere, Serv Bacteriol Virol, Assistance Publ Hop Paris, Fac M Hop Antoine Beclere Clamart France F-92141 istance Publ Hop Paris, Fac M
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 171, anno: 1999,
pagine: 127 - 132
SICI:
0378-1097(19990215)171:2<127:MCOACB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE; RESISTANCE; IMIPENEM; SEQUENCE; CLONING;
Keywords:
Chryseobacterium (Flavobacterium) indologenes; beta-lactamase; carbapenemase; metallo-beta-lactamase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Nordmann, P Hoparis,tre, Serv Bacteriol Virol, Fac Med Paris Sud, Assistance Publ Hop P Hop Bicetre 78 Rue Gen Leclerc Le Kremlin Bicetre France F-94275
Citazione:
S. Bellais et al., "Molecular characterization of a carbapenem-hydrolyzing beta-lactamase fromChryseobacterium (Flavobacterium) indologenes", FEMS MICROB, 171(2), 1999, pp. 127-132

Abstract

Chryseobacterium (Flavobacterium) indologenes 001 clinical strain was resistant to several beta-lactam classes including carbapenems. Shotgun cloningexperiments of Sau3AI restricted genomic DNA of C. indologenes 001 into pBKCMV cloning vector followed by transformation into Escherichia coli DH10B gave one recombinant plasmid possessing a 4.2-kb DNA insert. It encoded a pr 7.2 beta-lactamase of 239 amino acids (IND-I) which is a metallo-enzyme with a broad spectrum beta-lactam hydrolysis profile. This class B carbapenem-hydrolyzing beta-lactamase shares the highest identity (43%) with BlaB from C. meningosepticum, thus showing heterogeneity of carbapenem-hydrolyzingbeta-lactamases in Chryseobacterium spp. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:16:23