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Titolo:
Expression of the copper metallothionein CUPI from Saccharomyces cerevisiae in the cyanobacterium Synechococcus R2-PIM8(smtA)
Autore:
McCormick, PM; Cannon, GC; Heinhorst, S;
Indirizzi:
Univ So Mississippi, Dept Chem & Biochem, Hattiesburg, MS 39406 USA Univ So Mississippi Hattiesburg MS USA 39406 m, Hattiesburg, MS 39406 USA
Titolo Testata:
CURRENT MICROBIOLOGY
fascicolo: 3, volume: 38, anno: 1999,
pagine: 155 - 162
SICI:
0343-8651(199903)38:3<155:EOTCMC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANACYSTIS-NIDULANS R2; PROKARYOTIC METALLOTHIONEIN; ESCHERICHIA-COLI; YEAST METALLOTHIONEIN; GEL-ELECTROPHORESIS; METAL-BINDING; GENE; LOCUS; CADMIUM; TRANSFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Heinhorst, S Univ So Mississippi, Dept Chem & Biochem, Hattiesburg, MS 39406 USA Univ So Mississippi Hattiesburg MS USA 39406 g, MS 39406 USA
Citazione:
P.M. McCormick et al., "Expression of the copper metallothionein CUPI from Saccharomyces cerevisiae in the cyanobacterium Synechococcus R2-PIM8(smtA)", CURR MICROB, 38(3), 1999, pp. 155-162

Abstract

The coding sequence for Saccharomyces cerevisiae copper metallothionein (CUPI), the protein responsible for enhanced sequestration of Cu2+ in yeast, was placed under the control of an inducible synthetic Escherichia coli promoter in the cyanobacterial vector pTrcIK. Strain R2-PIM8(smtA) of Synechococcus sp. PCC 7942 was transformed with the resulting construct pMcK2, and the yeast CUPI gene was integrated into its genome via homologous recombination. The pMcK2 plasmid directed the synthesis of a protein product of the expected size in an in vitro E. coli transcription/translation system. In the transgenic cyanobacteria, the integrated CUPI gene was transcribed and produced a protein product with the expected metallothionein characteristics, as determined by Cd-109(2+) binding assays. At this level of expression, the yeast metallothionein, although functional, did not increase the tolerance range of the transgenic Synechococcus to Cu2+ Or Cd2+ beyond that of the untransformed R2-PIM8(smtA).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:18:42