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Titolo:
Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin
Autore:
Dvorakova, K; Dorr, RT; Valcic, S; Timmermann, B; Alberts, DS;
Indirizzi:
Univ Arizona, Arizona Canc Ctr, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 , Arizona Canc Ctr, Tucson, AZ 85724 USA
Titolo Testata:
CANCER CHEMOTHERAPY AND PHARMACOLOGY
fascicolo: 4, volume: 43, anno: 1999,
pagine: 331 - 335
SICI:
0344-5704(199904)43:4<331:POTGTD>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
(-)-EPIGALLOCATECHIN GALLATE; CELL-LINES; TUMOR INITIATION; SENCAR MICE; TANNIC-ACID; SKH-1 MICE; POLYPHENOLS; CARCINOGENESIS; INHIBITION; PROMOTION;
Keywords:
green tea; catechins; skin; topical; chemoprevention;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Dorr, RT Univ Arizona, Arizona Canc Ctr, 1515 N Campbell Ave, Tucson, AZ 85724 USA Univ Arizona 1515 N Campbell Ave Tucson AZ USA 85724 AZ 85724 USA
Citazione:
K. Dvorakova et al., "Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin", CANC CHEMOT, 43(4), 1999, pp. 331-335

Abstract

Purpose: (-)-Epigallocatechin gallate (EGCG), the main physiologically active polyphenol of green tea, is associated with antitumor and antimutagenicactivities. The goal of this study was to determine the stability and pharmacokinetic parameters of pure EGCG administered topically to human and mouse skin. Methods: EGCG was investigated by measuring drug levels of a 10% ointment formulation stored under different conditions over a period of 6 months. To determine pharmacokinetic parameters of EGCG following topical application, EGCG was applied as 10% EGCG in hydrophilic ointment USP to full-thickness mouse or human skin in vitro. The transdermal and intradermal. Penetration of EGCG was measured by reverse phase HPLC assays at different time-points. Results: The stability of EGCG in hydrophilic ointment USP was dependent on time, temperature and the degree of oxidation. For example. 10%EGCG was lost after 2 days at 37 degrees C, but the same formulation supplemented with 0.1% butylated hydroxytoluene (BHT) had significantly longer stability with greater than or equal to 90% EGCG remaining after 130 days at37 degrees C. Topical application of EGCG in hydrophilic ointment USP to human or mouse skin resulted in substantial intradermal uptake of up to 1-20% of the applied dose. However, transdermal penetration was observed only in mouse skin. Conclusion: The present study showed that topical applicationof EGCG in hydrophilic ointment USP achieved high concentrations in skin but negligible systemic availability. The drug was susceptible to oxidation,but if supplemented with BHT, the hydrophilic ointment formulation could potentially be used in clinical trials of skin cancer prevention.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:28:06