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Titolo:
Content of long-term culture-initiating cells, clonogenic progenitors and CD34(+) cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma
Autore:
Kasper, C; Ryder, WDJ; Durig, J; Nagesh, K; Scarffe, JH; Beelen, DW; Schaefer, UW; Chang, J; Testa, NG;
Indirizzi:
Christiegland, Canc Res Campaign, Dept Expt Haematol, Manchester, Lancs, En Christie Hosp Manchester Lancs England t Haematol, Manchester, Lancs, En ChristiedHosp, Canc Res Campaign, Dept Med Oncol, Manchester, Lancs, Englan Christie Hosp Manchester Lancs England Oncol, Manchester, Lancs, Englan Christie Hosp, Canc Res Campaign, Dept Med Stat, Manchester, Lancs, England Christie Hosp Manchester Lancs England Stat, Manchester, Lancs, England Univ Hosp Essen, Dept Bone Marrow Transplantat, Essen, Germany Univ Hosp Essen Essen Germany Bone Marrow Transplantat, Essen, Germany
Titolo Testata:
BRITISH JOURNAL OF HAEMATOLOGY
fascicolo: 2, volume: 104, anno: 1999,
pagine: 374 - 381
SICI:
0007-1048(199902)104:2<374:COLCCC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; COLONY-STIMULATING FACTOR; LIMITING DILUTION ASSAYS; PERIPHERAL-BLOOD; AUTOLOGOUS TRANSPLANTATION; BONE-MARROW; FUNCTIONAL-CHARACTERIZATION; FREQUENCY-ANALYSIS; CANCER-PATIENTS; CHEMOTHERAPY;
Keywords:
long-term culture-initiating cells; peripheral blood progenitor cells; CD34(+) cells; multiple myeloma; acute myeloid leukaemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kasper, C Univn,ssen Gesamthsch, Dept Med, Div Haematol, Hufelandstr 55, D-45122 Esse Univ Essen Gesamthsch Hufelandstr 55 Essen Germany D-45122 Esse
Citazione:
C. Kasper et al., "Content of long-term culture-initiating cells, clonogenic progenitors and CD34(+) cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma", BR J HAEM, 104(2), 1999, pp. 374-381

Abstract

Using a limiting dilution assay the frequency of lone-term culture-initiating cells (LTC-IC) in the apheresis products following mobilization by granulocyte-colony stimulating factor (G-CSF) with or without chemotherapy from14 normal donors (ND) for allogeneic bone marrow transplantation, 16 patients with multiple myeloma (MM) and 15 patients with acute myeloid leukaemia(AML), where the aphereses were intended for autologous transplantation. were compared. The estimated median incidences of LTC-IC in the first apheresis products from NU, MM and AML were 1/3289, 1/1775 and 1/13075 mononuclear cells (MNC)respectively. The patients with AML had a significantly lower incidence compared with the other two groups (P < 0.0001). There was a positive correlation between the incidence of LTC-IC and the number of CD34(+) cells, the number of GM-CFC. and the number of BFU-E. The positive association with GM-CFC or BFU-E was weaker. In these experiments the percentage of CD34(+) cells was the best predictor for the frequency of LTC-TC in the peripheral blood progenitor cells (PBPC). In eight cases of MM the LTC-IC assay was performed for both the first and second harvest. All cases had a lower LTC-IC frequency in the second harvest compared with the first, an average of 23% (13-42%,. 95% confidence interval) and this reduction was statistically significant (P < 0.001): CD34(+) cells were also lower (P < 0.001).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:41:02