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Titolo:
Phenotypic and functional evidence for the expression of CXCR4 receptor during megakaryocytopoiesis
Autore:
Riviere, C; Subra, F; Cohen-Solal, K; Cordette-Lagarde, V; Letestu, R; Auclair, C; Vainchenker, W; Louache, F;
Indirizzi:
Inst Gustave Roussy, INSERM, U362, F-94805 Villejuif, France Inst Gustave Roussy Villejuif France F-94805 , F-94805 Villejuif, France Inst Gustave Roussy, CNRS, URA 147, Villejuif, France Inst Gustave RoussyVillejuif France , CNRS, URA 147, Villejuif, France
Titolo Testata:
BLOOD
fascicolo: 5, volume: 93, anno: 1999,
pagine: 1511 - 1523
SICI:
0006-4971(19990301)93:5<1511:PAFEFT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HEMATOPOIETIC PROGENITOR CELLS; MARROW-BLOOD BARRIER; CHEMOKINE SDF-1; HIV-1 ENTRY; BONE-MARROW; PERIPHERAL-BLOOD; INFECTION; INVITRO; CD4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Louache, F Instillejuif,Roussy, INSERM, U362, PR1,39 Rue Camille Desmoulins, F-94805 V Inst Gustave Roussy PR1,39 Rue Camille Desmoulins Villejuif France F-94805
Citazione:
C. Riviere et al., "Phenotypic and functional evidence for the expression of CXCR4 receptor during megakaryocytopoiesis", BLOOD, 93(5), 1999, pp. 1511-1523

Abstract

The identification of stromal cell-derived factor (SDF)-1 alpha as a chemoattractant for human progenitor cells suggests that this chemokine and its receptor might represent critical determinants for the homing, retention, and exit of precursor cells from hematopoietic organs. In this study, we investigated the expression profile of CXCR4 receptor and the biological activity of SDF-1 alpha during megakaryocytopoiesis. CD34(+) cells from bone marrow and cord blood were purified and induced to differentiate toward the megakaryocyte lineage by a combination of stem-cell factor (SCF) and recombinant human pegylated megakaryocyte growth and development factor (PEG-rhuMGDF). After 6 days of culture, a time where mature and immature megakaryocytes were present, CD41(+) cells were immunopurified and CXCR4mRNA expression was studied. High transcript levels were detected by a RNase protection assay in cultured megakaryocytes derived from cord blood CD34(+) cells as wellas in peripheral blood platelets. The transcript levels were about equivalent to that found in activated T cells. By flow cytometry, a targe fraction(ranging from 30% to 100%) of CD41(+) cells showed high levels of CXCR4 antigen on their surface, its expression increasing in parallel with the CD41antigen during megakaryocytic differentiation. CXCR4 protein was also detected on peripheral blood platelets. SDF-1 alpha acts on megakaryocytes by inducing intracellular calcium mobilization and actin polymerization. In addition, in in vitro transmigration experiments, a significant proportion of megakaryocytes was observed to respond to this chemokine. This cell migration was inhibited by pertussis toxin, indicating coupling of this signal to heterotrimeric guanine nucleotide binding proteins. Although a close correlation between CD41a and CXCR4 expession was observed, cell surface markers as well as morphological criteria indicate a preferential attraction of immature megakaryocytes (low revel of CD41a and CD42a), suggesting that SDF-1 alpha is a potent attractant for immature megakaryocytic cells but is less active on fully mature megakaryocytes. This hypothesis was further supported by the observation that SDF-1 alpha induced the migration of colony forming unit-megakaryocyte progenitors (CFU-MK) and the expression of activation-dependent P-selectin (CD62P) surface antigen on early megakaryocytes, although no effect was observed on mature megakaryocytes and platelets. These results indicate that CXCR4 is expressed by human megakaryocytes and platelets. Furthermore, based on the tower responses of mature megakaryocytes and platelets to SDF-1 alpha as compared with early precursors, these data suggest a role for this chemokine in the maintenance and homing during early stages of megakaryocyte development. Moreover, because megakaryocytes are also reported to express CD4, it becomes important to reevaluate the rote of direct infection of these cells by the human immunodeficiency virus (HIV)-1 in HIV-1-related thrombocytopenia. (C) 1999 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 16:47:49