Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Optimization of single-cell gel electrophoresis (SCGE) for quantitative analysis of neuronal DNA damage
Autore:
Morris, EJ; Dreixler, JC; Cheng, KY; Wilson, PM; Gin, RM; Geller, HM;
Indirizzi:
Univscataway,nt New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Pi Univ Med & Dent New Jersey Piscataway NJ USA 08854 ch, Dept Pharmacol, Pi
Titolo Testata:
BIOTECHNIQUES
fascicolo: 2, volume: 26, anno: 1999,
pagine: 282 -
SICI:
0736-6205(199902)26:2<282:OOSGE(>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN CELLS; ALKALINE ELUTION; MAMMALIAN-CELLS; STRAND BREAKS; COMET ASSAY; REPAIR; APOPTOSIS; TOXICITY; MOMENT; TUMOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Geller, HM Univ5Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, 67 Univ Med & Dent New Jersey 675 Hoes Lane Piscataway NJ USA 08854
Citazione:
E.J. Morris et al., "Optimization of single-cell gel electrophoresis (SCGE) for quantitative analysis of neuronal DNA damage", BIOTECHNIQU, 26(2), 1999, pp. 282

Abstract

Neuronal death can be induced by DNA-damaging agents and occurs by apoptosis involving a specific signal-transduction pathway. However, to our knowledge, methods for the quantitative determination of DNA damage in individualneurons have trot yet been described. Here we optimize the single-cell gelelectrophoresis (SCGE) or "comet"-assay to measure DNA damage,within individual neurons growing in dissociated cell culture. In addition, Mle have,written a macro for the NIH Image program to determine the tail moment of individual comets. We have calibrated this method using gamma-irradiated (0-16Gy) cerebral cortical neurons from the rat central nervous system. Neuronal DNA damage (in the form of DNA strand breaks) occurs in a linear; dose-dependent manner, which can be quantitatively determined in. vitro using the SCGE assay. These data demonstrate that the SCGE assay is an effective method to measure DNA damage in individual neurons and may be highly useful forthe study of neuronal DNA damage formation, repair and apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:17:17