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Titolo:
Cloning and characterization of the transport modifier RS1 from rabbit which was previously assumed to be specific for Na+-D-glucose cotransport
Autore:
Reinhardt, J; Veyhl, M; Wagner, K; Gambaryan, S; Dekel, C; Akhoundova, A; Korn, T; Koepsell, H;
Indirizzi:
Univ Wurzburg, Inst Anat, D-8700 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-8700 nst Anat, D-8700 Wurzburg, Germany
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
fascicolo: 1, volume: 1417, anno: 1999,
pagine: 131 - 143
SICI:
0005-2736(19990204)1417:1<131:CACOTT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+/GLUCOSE COTRANSPORTER; SMALL-INTESTINE; MESSENGER-RNA; EXPRESSION; PROTEIN; LOCALIZATION; SGLT1; GENE; CDNA; DNA;
Keywords:
transport modifier RS1; primary structure; expression; Na+-D-glucose cotransporter SGLT1; organic cation transporter OCT2; rabbit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Koepsell, H Univ Wurzburg, Inst Anat, Koellikerstr 6, D-8700 Wurzburg, Germany Univ Wurzburg Koellikerstr 6 Wurzburg Germany D-8700 Germany
Citazione:
J. Reinhardt et al., "Cloning and characterization of the transport modifier RS1 from rabbit which was previously assumed to be specific for Na+-D-glucose cotransport", BBA-BIOMEMB, 1417(1), 1999, pp. 131-143

Abstract

previously we cloned membrane associated polypeptides from pig and man (pRS1, hRS1) which altered rate and glucose dependence of Na+-D-glucose cotransport expressed by SGLT1 from rabbit and man. This paper describes the cloning of a related cDNA sequence from rabbit intestine (rbRS1) which encodes a gene product with about 65% amino acid identity to pRS1 and hRS1. Hybridization of endonuclease-restricted genomic DNA with cDNA fragments of rbRS1 showed that there is only one gene with similarity to rbRS1 in rabbit, and genomic PCR amplifications revealed that the rbRS1 gene is intronless, Comparing the transcription of rbRS1 and rbSGLT1 in various tissues and cell types, different mRNA patterns were obtained for both genes. In Xenopus oocytes the V-max of expressed Na+-D-glucose cotransport was increased or decreased when rbRS1 was coexpressed with rbSGLT1 or hSGLT1, respectively. After coexpression with hSGLT1 the glucose dependence of the expressed transport was changed. By coexpression of rbRS1 with the human organic cation transporter hOCT2 the expressed cation uptake was not altered; however, the expressed cation uptake was drastically decreased when hRS1 was coexpressed with hOCT2. The data show that RS1 can modulate the function of transporters with nonhomologous primary structures. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:39:15