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Titolo:
Cardiac impairment and nitric oxide synthase activity in the chronic portal vein-stenosed rat
Autore:
Battarbee, HD; Zavecz, JH; Grisham, MB; Maloney, RE; Chandler, LJ; Mercer, JW; Cady, FM;
Indirizzi:
Louisiana71130e Univ, Med Ctr, Dept Cellular & Mol Physiol, Shreveport, LALouisiana State Univ Shreveport LA USA 71130 Mol Physiol, Shreveport, LA Louisiana State Univ, Med Ctr, Dept Pharmacol, Shreveport, LA 71130 USA Louisiana State Univ Shreveport LA USA 71130 ol, Shreveport, LA 71130 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 2, volume: 39, anno: 1999,
pagine: G363 - G372
SICI:
0193-1857(199902)39:2<G363:CIANOS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORCE-FREQUENCY-RELATIONSHIP; HYPERTENSIVE RATS; HYPERDYNAMIC CIRCULATION; CIRRHOTIC RATS; PERIPHERAL VASODILATION; SODIUM RETENTION; L-ARGININE; IN-VITRO; ENDOTOXIN; MYOCYTES;
Keywords:
endotoxin; cirrhosis; liver disease; heart; constitutive nitric oxide synthase; calcium; contractility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Battarbee, HD Louisianaay,ate Univ, Med Ctr, Dept Cellular & Mol Physiol, 501 Kings Highw Louisiana State Univ 501 Kings Highway Shreveport LA USA 71130
Citazione:
H.D. Battarbee et al., "Cardiac impairment and nitric oxide synthase activity in the chronic portal vein-stenosed rat", AM J P-GAST, 39(2), 1999, pp. G363-G372

Abstract

Decreased cardiac contractility and beta-adrenergic responses have been observed in the chronic portal vein-stenosed (PVS) rat. Because nitric oxide (NO) may be increased in PVS and has been recognized as a negative inotropic agent, we investigated the induction of NO synthase (NOS2) and/or changesin constitutive NOS (NOS3) as factors in the cardiac dysfunction of the PVS rat. Ten to twelve days after portal vein stenosis or sham operation, cardiac function was evaluated in paced left ventricular papillary muscles (LVPM) and right ventricular strips (RV). To determine if NO modulation of contractile function was altered in PVS, we examined the increase in developedtension produced by the effect of N-omega-nitro-L-arginine (L-NNA) on the myocardial force-frequency relationship. Cardiac tissue NOS2 and NOS3 activities were assayed, Western blot analyses of NOS2 and NOS3 expression were performed, and circulating nitrate-nitrite (NOX) levels (an indicator of invivo NOS activity) were assayed. Basal LVPM and RV contractile indexes were significantly reduced in PVS (30-50%), without a change in the relaxationrate. No between-group differences in the cardiac NOS2 or NOS3 enzymatic activities of PVS and sham-operated (SO) rats were observed. Western blots revealed no cardiac NOS2 expression in either SO or PVS rats. In contrast, NOS3 was expressed in both SO and PVS rats, but there was no quantitative difference in expression between the two groups. Changes in the cardiac force-frequency relationship (staircase effect) after L-NNA were consistent withNOS3 modulation of contractile function in both SO and PVS rats, but therewas no between-group difference in the modulation. Circulating NOX concentrations did not differ between SO and PVS rats. In conclusion, protein expression data, enzymatic assays, end-product assays, and functional data indicate that between-group differences in NOS2 and NOS3 activity are not responsible for the cardiac impairment that has been observed in the chronic PVSrat.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:11:23