Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of c-Src tyrosine kinase on ethanol sensitivity of recombinant NMDA receptors expressed in HEK 293 cells
Autore:
Anders, DL; Blevins, T; Sutton, G; Chandler, LJ; Woodward, JJ;
Indirizzi:
Virginiacol,monwealth Univ, Med Coll Virginia Campus, Dept Pharmacol & Toxi Virginia Commonwealth Univ Richmond VA USA 23298 s, Dept Pharmacol & Toxi Louisiana State Univ, Coll Med, Dept Pharmacol, Shreveport, LA 71105 USA Louisiana State Univ Shreveport LA USA 71105 ol, Shreveport, LA 71105 USA
Titolo Testata:
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
fascicolo: 2, volume: 23, anno: 1999,
pagine: 357 - 362
SICI:
0145-6008(199902)23:2<357:EOCTKO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-ASPARTATE RECEPTORS; HIPPOCAMPAL-NEURONS; INHIBITION; PROTEIN; EXPOSURE; CHANNELS; CURRENTS; CALCIUM;
Keywords:
N-methyl-D-aspartate; tyrosine phosphorylation; ethanol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Anders, DL Virginiacol,monwealth Univ, Med Coll Virginia Campus, Dept Pharmacol & Toxi Virginia Commonwealth Univ Box 980524 Richmond VA USA 23298 oxi
Citazione:
D.L. Anders et al., "Effects of c-Src tyrosine kinase on ethanol sensitivity of recombinant NMDA receptors expressed in HEK 293 cells", ALC CLIN EX, 23(2), 1999, pp. 357-362

Abstract

N-Methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that are important mediators of the actions of the excitatory amino acid neurotransmitter glutamate. Previous studies have shown that ethanol inhibits the function of both wild-type receptors found in neurons and recombinant NMDA receptors expressed in heterologous cells, such as oocytes and transfected mammalian cells. Although some studies have reported that certain subunit combinations display an enhanced sensitivity to ethanol, this effect is not observed in all experimental systems. This discrepancy may be due to varyinglevels of endogenous modulators, such as kinases, between different cell preparations. In this study, we investigated the effects of tyrosine phosphorylation on the ethanol sensitivity of NMDA receptor function using a recombinant cell system where levels of both NMDA subunits and protein kinases can be more carefully controlled. Human embryonic kidney (HEK 293) cells were transfected with different NMDA receptor subunits and a c-Src-green fluorescent protein (GFP) fusion protein that could be directly visualized in living cells. Agonist-stimulated calcium flux was measured in single cells using fura-2 video imaging, As expected, cells transfected with the NR1/NR2B subunits were more sensitive to inhibition by the NR2 selective antagonist ifenprodil than those transfected with NR1/ NR2A or NR1/NR2A/NR2B subunits. All receptor combinations were inhibited by ethanol (25 and 100 mM), with the NR1/NR2B combination being slightly more sensitive than NR1/NR2A or NR1/NR2A/ NR2B, Control and NMDA-receptor transfected HEK 293 cells displayed a low degree of tyrosine phosphorylation as measured by immunofluorescence and Western immunoblotting using an antiphosphotyrosine antibody. Phosphorylation was markedly enhanced in cells transfected with the c-Src-GFP fusionprotein. The sensitivity of NMDA receptors to either 25 or 100 mM ethanol,or 10 mu M ifenprodil, was not significantly altered by co-transfection with c-Src-GFP, These results indicate that, although NMDA receptors can be atarget of c-Src tyrosine kinase, tyrosine phosphorylation by this enzyme does not modulate the inhibitory effects of ethanol on NMDA-activated currents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:33:22