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Titolo:
DETECTION, IDENTIFICATION AND SEMI-QUANTIFICATION OF MALARIA PARASITES BY NASBA AMPLIFICATION OF SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCES
Autore:
SMITS HL; GUSSENHOVEN GC; TERPSTRA W; SCHUKKINK RAF; VANGEMEN B; VANGOOL T;
Indirizzi:
ROYAL TROP INST,DEPT BIOMED RES,MEIBERGDREEF 39 NL-1105 AZ AMSTERDAM NETHERLANDS ORGANON TEKNIKA NV NL-5281 RM BOXTEL NETHERLANDS UNIV AMSTERDAM,ACAD MED CTR,PARASITOL LAB,DEPT MED MICROBIOL NL-1105 AZ AMSTERDAM NETHERLANDS
Titolo Testata:
Journal of microbiological methods
fascicolo: 1, volume: 28, anno: 1997,
pagine: 65 - 75
SICI:
0167-7012(1997)28:1<65:DIASOM>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROTITER PLATE HYBRIDIZATION; PLASMODIUM-FALCIPARUM; INFECTION; GENES;
Keywords:
MALARIA; MOLECULAR DETECTION; NASBA; RNA AMPLIFICATION; QUANTIFICATION; PARASITEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H.L. Smits et al., "DETECTION, IDENTIFICATION AND SEMI-QUANTIFICATION OF MALARIA PARASITES BY NASBA AMPLIFICATION OF SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCES", Journal of microbiological methods, 28(1), 1997, pp. 65-75

Abstract

We have adapted the Nucleic Acid Sequence Based Amplification method (NASBA), a molecular detection method for the amplification of specific RNA sequences, for the detection, identification and semi-quantification of malaria parasites. Primers and probes were selected based on the nucleotide sequence of the small subunit ribosomal RNA gene. NASBA enabled the detection of as little as 0.01 parasitized erythrocytes per mu l blood. When applied to blood samples of patients, NASBA allowedthe detection of malaria parasites in confirmed malaria patients. in addition, malaria parasites were detected in a number of patients witha history of suspected malaria. Semi-quantification over a wide parasite range was achieved by analysing serial end-point dilutions of RNA preparations, Semi-quantitative NASBA analysis of malaria parasites infollow-up samples of treated malaria patients showed a rapid decline of parasite levels in most patients and a low level of persistent parasites in some of the patients. (C) 1997 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:15:57