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Titolo:
Genetic heterogeneity of porcine and ruminant pestiviruses mainly isolatedin Japan
Autore:
Sakoda, Y; Ozawa, S; Damrongwatanapokin, S; Sato, M; Ishikawa, K; Fukusho, A;
Indirizzi:
Natl Inst Anim Hlth, Dept Exot Dis, Immunol Lab, Tokyo 1870022, Japan NatlInst Anim Hlth Tokyo Japan 1870022 mmunol Lab, Tokyo 1870022, Japan Nippon Vet & Anim Sci Univ, Tokyo 1808602, Japan Nippon Vet & Anim Sci Univ Tokyo Japan 1808602 niv, Tokyo 1808602, Japan Natl Inst Anim Hlth, Bangkok 10900, Thailand Natl Inst Anim Hlth BangkokThailand 10900 Hlth, Bangkok 10900, Thailand Tochigi Anim Hyg Lab, Utsunomiya, Tochigi 3200056, Japan Tochigi Anim Hyg Lab Utsunomiya Tochigi Japan 3200056 higi 3200056, Japan Natl Vet Assay Lab, Assay Div 1, Tokyo 1858511, Japan Natl Vet Assay Lab Tokyo Japan 1858511 Assay Div 1, Tokyo 1858511, Japan
Titolo Testata:
VETERINARY MICROBIOLOGY
fascicolo: 1, volume: 65, anno: 1999,
pagine: 75 - 86
SICI:
0378-1135(19990223)65:1<75:GHOPAR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SWINE FEVER VIRUS; HOG-CHOLERA VIRUS; VIRAL DIARRHEA VIRUS; POLYMERASE CHAIN-REACTION; BORDER DISEASE VIRUS; MONOCLONAL-ANTIBODIES; NUCLEOTIDE-SEQUENCE; MOLECULAR-CLONING; CATTLE; PIGS;
Keywords:
border disease virus; bovine viral diarrhoea virus; classical swine fever virus; pestivirus; phylogenetic analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Sakoda, Y Natl Inst Anim Hlth, Dept Exot Dis, Immunol Lab, 6-20-1 Josui-honcho, Tokyo Natl Inst Anim Hlth 6-20-1 Josui-honcho Tokyo Japan 1870022 kyo
Citazione:
Y. Sakoda et al., "Genetic heterogeneity of porcine and ruminant pestiviruses mainly isolatedin Japan", VET MICROB, 65(1), 1999, pp. 75-86

Abstract

The genetic variability of porcine and ruminant pestiviruses was studied by comparative nucleotide sequence analysis of 73 isolates (42 porcine and 31 ruminant), including 65 Japanese isolates (35 porcine and 30 ruminant), The 5'-untranslated region (UTR) amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) was determined by direct sequencing and phylogenetic analysis was performed from the nucleotide sequence data. Most porcineisolates were divided into two major subgroups, classical swine fever virus (CSFV) subgroup 1 (CSFV-1, represented by Brecia strain) and subgroup 2 (CSFV-2, represented by Alfort strain). However, the Japanese Kanagawa/74, Okinawa/86, Okinawa/86-2 and Thai CBR/93 strains were the most distinct variants and these were assigned to another new disparate subgroup, CSFV-3 (represented by p97 strain). Most ruminant isolates were classified as the bovine viral diarrhoea virus (BVDV) genotype-I (BVDV-I) and subdivided into twosubgroups, BVDV-Ia (represented by the NADL strain) and Ib (represented bythe Osloss strain). Two bovine isolates (MS-1 and SY-89) and a contaminating strain (V/FLL) from an ovine cell line were classified as BVDV genotype-II (BVDV-II) on genetic characteristics. These data suggested that the detection and phylogenetic analysis of 5'-UTRs are useful for the rapid characterization of field isolates. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 15/07/20 alle ore 19:17:51