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Titolo:
The detection of chronic heart graft rejection by P-31 NMR spectroscopy
Autore:
Suzuki, K; Hamano, K; Ito, H; Fujimura, Y; Esato, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Surg 1, Ube, Yamaguchi 755, Japan Yamaguchi Univ Ube Yamaguchi Japan 755 Surg 1, Ube, Yamaguchi 755, Japan
Titolo Testata:
SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY
fascicolo: 2, volume: 29, anno: 1999,
pagine: 143 - 148
SICI:
0941-1291(1999)29:2<143:TDOCHG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; CARDIAC ALLOGRAFT-REJECTION; TRANSPLANTATION; MARKER; RAT;
Keywords:
P-31 nuclear magnetic resonance spectroscopy; chronic rejection; heart transplantation; graft arteriosclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hamano, K Yamaguchianniv, Sch Med, Dept Surg 1, 1144 Kogushi, Ube, Yamaguchi 755, Jap Yamaguchi Univ 1144 Kogushi Ube Yamaguchi Japan 755 chi 755, Jap
Citazione:
K. Suzuki et al., "The detection of chronic heart graft rejection by P-31 NMR spectroscopy", SURG TODAY, 29(2), 1999, pp. 143-148

Abstract

The usefulness of phosphorus-31 nuclear magnetic resonance spectroscopy (P-31 NMRS) for detecting heart graft rejection after transplantation has been investigated by several researchers, and it has thus been demonstrated tobe a valid technique for detecting acute myocardial rejection. In this study, we investigated the value of P-31 NMRS to assess chronic cardiac allograft rejection. Lewis rat hearts were transplanted into the femoral region of F-344 rat recipients which were treated with cyclosporine, 5 mg/kg body weight, by a daily intramuscular injection for 30 days beginning on the day of transplantation. The control isografts employed Lewis donors and recipients not given cyclosporine. The ratios of phosphocreatine (PCr) to inorganic phosphate (P-i), beta-adenosine triphosphate (beta-ATP) to P-i, and PCr to beta-ATP were monitored using surface coil P-31 NMRS, P-31 NMRS was performed 3, 30, and 60 days after transplantation, and the degree of the rejection and arteriosclerosis of the coronary arteries were then assessed histologically. The PCr:P-i and beta-ATP:P-i ratios for the allografts demonstrated a significant decrease on postoperative day (POD) 60 from that on POD 30(PCr:P-i, P < 0.001; beta-ATP:P-i, P < 0.01). Although a significant difference existed between the isografts and allografts on POD 60 (PCr:P-i, P < 0.01; beta-ATP:P-i, P < 0.01), no significant difference was found in the PCr:beta-ATP ratio between the allografts and the isografts, On POD 60, the allografts showed significant graft rejection and arteriosclerotic changes in the coronary arteries. These findings therefore demonstrated the effectiveness of P-31 NMRS for detecting chronic graft rejection in a rat model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:57:12