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Titolo:
An examination of coaxial stacking of helical stems in a pseudoknot motif:The gene 32 messenger RNA pseudoknot of bacteriophage T2
Autore:
Holland, JA; Hansen, MR; Du, ZH; Hoffman, DW;
Indirizzi:
Univ Texas, Dept Chem & Biochem, Inst Cell & Mol Biol, Austin, TX 78712 USA Univ Texas Austin TX USA 78712 Inst Cell & Mol Biol, Austin, TX 78712 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 2, volume: 5, anno: 1999,
pagine: 257 - 271
SICI:
1355-8382(199902)5:2<257:AEOCSO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENYLALANINE TRANSFER-RNA; RIBOSOMAL-PROTEIN S15; NITROGEN-15-LABELED OLIGODEOXYNUCLEOTIDES; NMR-SPECTROSCOPY; N-15 NMR; LABELED RIBONUCLEOTIDES; ESCHERICHIA-COLI; REGULATORY SITE; CHEMICAL-SHIFT; READ-THROUGH;
Keywords:
NMR spectroscopy; RNA pseudoknot; RNA structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffman, DW Univ Texas, Dept Chem & Biochem, Inst Cell & Mol Biol, Austin,TX 78712 USA Univ Texas Austin TX USA 78712 Mol Biol, Austin, TX 78712 USA
Citazione:
J.A. Holland et al., "An examination of coaxial stacking of helical stems in a pseudoknot motif:The gene 32 messenger RNA pseudoknot of bacteriophage T2", RNA, 5(2), 1999, pp. 257-271

Abstract

The RNA pseudoknot located at the 5' end of the gene 32 messenger RNA of bacteriophage T2 contains two A-form helical stems connected by two loops, in an H-type pseudoknot topology. A combination of multidimensional NMR methods and isotope labeling were used to investigate the pseudoknot structure,resulting in a more detailed structural model than provided by earlier homonuclear NMR studies. Of particular significance, the interlace between thestacked helical stems within the pseudoknot motif is described in detail. The two stems are stacked in a coaxial manner, with an approximately 18 degrees rotation of stem1 relative to stem2 about an axis that is parallel to the helical axis. This rotation serves to relieve what would otherwise be arelatively close phosphate-phosphate contact at the junction of the two stems, while preserving the stabilizing effects of base stacking. The abilityof the NMR data to determine pseudoknot bending was critically assessed. The data were found to be a modestly precise indicator of pseudoknot bending, with the angle between the helical axes of stem1 and stem:! being in the range of 15 +/- 15 degrees. Pseudoknot models with bend angles within this range are equally consistent with the data, since they differ by only smallamounts in the relatively short-range interproton distances from which thestructure was derived. The gene 32 messenger RNA pseudoknot was compared with other RNA structures with coaxial or near-coaxial stacked helical stems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:36:37