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Titolo:
Purification of the trehalase GMTRE1 from soybean nodules and cloning of its cDNA. GMTRE1 is expressed at a low level in multiple tissues
Autore:
Aeschbacher, RA; Muller, J; Boller, T; Wiemken, A;
Indirizzi:
Univ Basel, Inst Bot, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 nst Bot, CH-4056 Basel, Switzerland
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 2, volume: 119, anno: 1999,
pagine: 489 - 495
SICI:
0032-0889(199902)119:2<489:POTTGF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT-NODULES; YEAST; GENE; PLANTS; THERMOTOLERANCE; ARABIDOPSIS; ACQUISITION; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Aeschbacher, RA Univ Basel, Inst Bot, Hebelstr 1, CH-4056 Basel, Switzerland Univ Basel Hebelstr 1 Basel Switzerland CH-4056 itzerland
Citazione:
R.A. Aeschbacher et al., "Purification of the trehalase GMTRE1 from soybean nodules and cloning of its cDNA. GMTRE1 is expressed at a low level in multiple tissues", PLANT PHYSL, 119(2), 1999, pp. 489-495

Abstract

Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranoside), a disaccharide widespread among microbes and lower invertebrates, is generally believed to be nonexistent in higher plants. However, the recent discovery of Arabidopsis genes whose products are involved in trehalose synthesis has renewed interest in the possibility of a function of trehalose in higher plants. We previously showed that trehalase, the enzyme that degrades trehalose, is present in nodules of soybean (Glycine max [L.] Merr.), and we characterized the enzyme as an apoplastic glycoprotein. Here we describe the purification of this trehalase to homogeneity and the cloning of a full-length cDNAencoding this enzyme, named GMTRE1 (G. max trehalase 1). The amino acid sequence derived from the open reading frame of GMTRE1 shows strong homology to known trehalases from bacteria, fungi, and animals. GMTRE1 is a single-copy gene and is expressed at a low but constant level in many tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 19:49:37