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Titolo:
Tyrosine kinase involvement in apamin-sensitive inhibitory responses of rat distal colon
Autore:
Takeuchi, T; Kishi, M; Hirayama, N; Yamaji, M; Ishii, T; Nishio, H; Hata, F; Takewaki, T;
Indirizzi:
UnivJapana Prefecture, Coll Agr, Dept Vet Pharmacol, Sakai, Osaka 5998531,Univ Osaka Prefecture Sakai Osaka Japan 5998531 ol, Sakai, Osaka 5998531, Univem,aka Prefecture, Res Inst Adv Sci & Technol, Dept Mol Physiol & Bioch Univ Osaka Prefecture Sakai Osaka Japan 5998531 Dept Mol Physiol & Bioch Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan Gifu Univ Gifu Japan 5011193 nited Grad Sch Vet Sci, Gifu 5011193, Japan
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 1, volume: 514, anno: 1999,
pagine: 177 - 188
SICI:
0022-3751(19990101)514:1<177:TKIIAI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLASE-ACTIVATING PEPTIDE; SMOOTH-MUSCLE CELLS; VASOACTIVE-INTESTINAL-PEPTIDE; KV1.3 POTASSIUM CHANNEL; GUINEA-PIG CECUM; ADENYLATE-CYCLASE; K+ CHANNELS; PROTEIN-KINASE; NITRIC-OXIDE; TENIA-COLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Takeuchi, T UnivJapana Prefecture, Coll Agr, Dept Vet Pharmacol, Sakai, Osaka 5998531, Univ Osaka Prefecture Sakai Osaka Japan 5998531 saka 5998531,
Citazione:
T. Takeuchi et al., "Tyrosine kinase involvement in apamin-sensitive inhibitory responses of rat distal colon", J PHYSL LON, 514(1), 1999, pp. 177-188

Abstract

1. It has been suggested that pituitary adenylate cyclase activating peptide (PACAP) may be involved in the non-adrenergic, non-cholinergic (NANC) inhibitory response of longitudinal muscle of rat distal colon. In this study, we have investigated the intracellular mechanism of PACAP-induced relaxation in this muscle.2. PACAP induced an apamin-sensitive relaxation of the longitudinal muscle. The tyrosine kinase inhibitors genistein at 10 mu M and tyrphostin 25 at 30 mu M, but not the cyclic AMP-dependent protein kinase inhibitor R-p-8-bromoadenosine-3',5'-cyclic monophosphorothioate at 30 mu M significantly inhibited the PACAP-induced relaxation to 60% and 25% of control values, respectively. PACAP did not increase the cyclic AMP content of the muscle.3. Tyrphostin 25 at 10 mu M significantly inhibited the relaxation of longitudinal muscle induced by electrical field stimulation (EFS), to 50% of control values. Apamin at 1 mu M, an antagonist of small conductance Ca2+-activated K+ channels, also inhibited the relaxation, to 42% of control values. The inhibitory effects of tyrphostin 25 and apamin were not additive (44%of control values).4. PACAP induced an apamin-sensitive, slow hyperpolarization of the cell membrane of the muscle. Tyrphostin 25 at 3 mu M inhibited this PACAP-inducedhyperpolarization. Tyrphostin 25 at 10 mu M and genistein at 10 mu M inhibited the apamin-sensitive inhibitory junction potentials induced by a single pulse of EPS.5. The PACAP-induced relaxation of longitudinal muscle occurred with a concomitant decrease in intracellular Ca2+ levels ([Ca2+](i)). Tyrphostin 25 at 10 mu M and apamin at 1 mu M abolished these PACAP-induced responses.6. From these findings it is suggested that the activation of tyrosine kinase is involved in PACAP-induced relaxation of longitudinal muscle from ratdistal colon, 'upstream of' the activation of apamin-sensitive K+ channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:53:29