Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Rapid clearance of transplanted hepatocytes from pulmonary capillaries in rats indicates a wide safety margin of liver repopulation and the potentialof using surrogate albumin particles for safety analysis
Autore:
Rajvanshi, P; Fabrega, A; Bhargava, KK; Kerr, A; Pollak, R; Blanchard, J; Palestro, CJ; Gupta, S;
Indirizzi:
YeshivaNYniv Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Bronx,Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 Res Ctr, Bronx, YeshivaNYniv Albert Einstein Coll Med, Dept Med, Div Gastroenterol, Bronx,Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 oenterol, Bronx, Yeshiva Univ Albert Einstein Coll Med, Dept Radiol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Div Nucl Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Univ Iowa Hosp & Clin, Dept Surg, Iowa City, IA 52242 USA Univ Iowa Hosp &Clin Iowa City IA USA 52242 urg, Iowa City, IA 52242 USA Univ Illinois, Dept Surg, Chicago, IL 60680 USA Univ Illinois Chicago IL USA 60680 nois, Dept Surg, Chicago, IL 60680 USA
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 2, volume: 30, anno: 1999,
pagine: 299 - 310
SICI:
0168-8278(199902)30:2<299:RCOTHF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROAGGREGATED ALBUMIN; GENE-THERAPY; HEPATOCELLULAR TRANSPLANTATION; PORTAL HEMODYNAMICS; HEPATIC-ARTERY; CIRRHOSIS; SHUNTS; CELLS; FAILURE; HYPERTENSION;
Keywords:
hepatocyte; liver; lungs; macroaggregated albumin; transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Gupta, S Yeshivarrisv Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, 1300 Mo Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave,Ulmann 625 Bronx NY USA 10461
Citazione:
P. Rajvanshi et al., "Rapid clearance of transplanted hepatocytes from pulmonary capillaries in rats indicates a wide safety margin of liver repopulation and the potentialof using surrogate albumin particles for safety analysis", J HEPATOL, 30(2), 1999, pp. 299-310

Abstract

Background/Aims: Applications of liver repopulation by hepatocyte transplantation require analysis of cell biodistributions, particularly when portasystemic shunting coexists. The aims of this study were to determine the fate of hepatocytes transplanted into the pulmonary vascular bed and to examine whether cell biodistributions could be approximated by convenient surrogates. Methods: Rat hepatocytes and macroaggregated serum albumin particles of similar sizes were injected into the portal and pulmonary vascular beds of rats, followed by biodistribution, survival and function analyses. Results: Although functionally intact, virtually all hepatocytes were cleared from the pulmonary capillaries within 24 h, Serum albumin levels increased minimally in Nagase analbuminemic rats with or without portacaval shunting to enhance delivery of portal factors to transplanted cells in lungs. Despite intravenous injection of hepatocytes approaching >1x10(9) cells in humans, the hemodynamic changes were limited to transient increases in rightatrial pressures, The hepatocyte distributions in specific vascular beds were largely reproduced by macroaggregated human serum albumin particles. Conclusions: Incidental intrapulmonary cell translocations during liver repopulation will have a wide safety margin, Use of macroaggregated serum albumin particles as surrogates for initial short-term biodistribution and safety analysis will advance hepatocyte transplantation, as the cost of GLP-certified laboratories and consumption of scarce donor livers will be avoided.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 11:17:30