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Titolo:
Cellular distribution and ontogeny of insulin-like growth factors (IGFs) and IGF binding protein messenger RNAs and peptides in developing rat pancreas
Autore:
Hill, DJ; Hogg, J; Petrik, J; Arany, E; Han, VKM;
Indirizzi:
St Josephs Hlth Ctr, Lawson Res Inst, London, ON N6A 4V2, Canada St Josephs Hlth Ctr London ON Canada N6A 4V2 , London, ON N6A 4V2, Canada Univ Western Ontario, Dept Physiol, London, ON N6A 5A5, Canada Univ Western Ontario London ON Canada N6A 5A5 London, ON N6A 5A5, Canada Univ Western Ontario, Dept Med, London, ON N6A 5A5, Canada Univ Western Ontario London ON Canada N6A 5A5 London, ON N6A 5A5, Canada Univ Western Ontario, Dept Paediat, London, ON N6A 5A5, Canada Univ Western Ontario London ON Canada N6A 5A5 London, ON N6A 5A5, Canada Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5A5, Canada Univ Western Ontario London ON Canada N6A 5A5 London, ON N6A 5A5, Canada Univ Western Ontario, Dept Obstet & Gynaecol, London, ON N6A 5A5, Canada Univ Western Ontario London ON Canada N6A 5A5 London, ON N6A 5A5, Canada
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 2, volume: 160, anno: 1999,
pagine: 305 - 317
SICI:
0022-0795(199902)160:2<305:CDAOOI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-FETAL PANCREAS; I GENE-EXPRESSION; SOMATOMEDIN-C; NEONATAL RAT; BETA-CELLS; ISLETS; LOCALIZATION; RECEPTORS; CULTURE; PANCREATECTOMY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Hill, DJ St Josephs Hlth Ctr, Lawson Res Inst, 268 Grosvenor St, London, ON N6A 4V2, St Josephs Hlth Ctr 268 Grosvenor St London ON Canada N6A 4V2 V2,
Citazione:
D.J. Hill et al., "Cellular distribution and ontogeny of insulin-like growth factors (IGFs) and IGF binding protein messenger RNAs and peptides in developing rat pancreas", J ENDOCR, 160(2), 1999, pp. 305-317

Abstract

To determine the role of insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) in the development of the pancreas, and specifically of the islets of Langerhans, we have examined the cellular distribution anddevelopmental changes in the expression of IGFs and IGFBPs in the pancreasof the fetal and neonatal rat between 19.5 days of gestation and postnatalday 28. This represents a period of substantial growth and restructuring of the beta cell component in islets of this species. IGF-I, IGF-II, and IGFBPs-1 to -6 mRNAs were localized by in situ hybridization, and peptides by immunohistochemistry, in histological sections. IGF-II mRNA was highly expressed in islet cells and some ductal epithelial cells in late fetal and early neonatal life, but was barely detectable by postnatal day 28. IGF-II peptide showed a similar distribution. IGF-I mRNA was barely detected in the fetus or neonate and was localized predominantly in the ductal and acinar tissues after postnatal day 7. IGF-I immunoreactivity was associated with some islet cells in the fetus and neonate, suggesting an endocrine rather thana paracrine source. We performed co-localization studies to assess whetherthe distribution of IGFs within the pancreas might be due to a sequestration by locally produced IGFBPs, The presence of mRNAs for both IGFBPs-1 and -2 was minimal in the pancreas prior to postnatal day 7, although subsequently IGFBP-1 mRNA was seen in islet cells, while IGFBP-2 mRNA was localized in both islets and acinar tissues. In contrast, both IGFBPs-1 and -2 immunoreactivities were identified in islets from late fetal life, suggesting a circulatory source for these IGFBPs during early pancreatic development. IGFBPs-3 to -5 mRNAs and immunoreactivities were identified within islet cellsthroughout fetal and neonatal life, with IGFBPs-3 and -5 being mainly associated with the alpha cell-rich islet mantle. The results show a compartmentalization of IGFs within pancreatic tissue, reflecting both paracrine and endocrine sources. The localization and action of IGFs in pancreas likely involves sequestration and distribution by endogenous as well as circulatingIGFBPs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:48:16