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Titolo:
Urinary excretion of 19-norandrosterone of endogenous origin in man: quantitative analysis by gas chromatography mass spectrometry
Autore:
Dehennin, L; Bonnaire, Y; Plou, P;
Indirizzi:
Lab Federat Natl Courses Francaises, F-92290 Chatenay Malabry, France Lab Federat Natl Courses Francaises Chatenay Malabry France F-92290 ance
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 721, anno: 1999,
pagine: 301 - 307
SICI:
1387-2273(19990122)721:2<301:UEO1OE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOTOPE-DILUTION; 19-NORTESTOSTERONE; ESTROGENS; PLASMA;
Keywords:
19-norandrosterone; steroid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Dehennin, L LabMalabry, Natl Courses Francaises, 169 Ave Div Leclerc, F-92290 Chatenay Lab Federat Natl Courses Francaises 169 Ave Div Leclerc Chatenay Malabry France F-92290
Citazione:
L. Dehennin et al., "Urinary excretion of 19-norandrosterone of endogenous origin in man: quantitative analysis by gas chromatography mass spectrometry", J CHROMAT B, 721(2), 1999, pp. 301-307

Abstract

A GC-MS method, using deuterium-labelled 19-noretiocholanolone as internalstandard and following an extensive LC purification prior to selected ion monitoring of the bis(trimethylsilyl) ethers at ion masses m/z 405, 419, 420 and 421, allowed the quantitation of subnanogram amounts of 19-norandrosterone present in 10-ml urine samples at mit 405. Thirty healthy men, free of anabolic androgen supply, delivered 24-h urine collections in 4 timed fractions. Accuracy was proven by the equation, relating added (0.05-1 ng/ml) to measured analyte, which had a slope not significantly different from 1. Precision (RSD) was 4% at a concentration of 0.4 ng/ml, and 14% at 0.04 ng/ml. Analytical recovery was 82%. The limit of quantitation was 0.02 ng/ml. The excretion ranges were 0.03-0.25 mu g/24 h or 0.01-0.32 ng/ml in nonfractionated 24-h urine. Taking into account inter-individual variability and log-normal distribution, a threshold of 19-norandrosterone endogenous concentration of 2 ng/ml, calculated as the geometric mean plus 4 SD, was established. This value corresponds to the decision limit advised by sport authorities for declaring positive (anabolic) doping with nandrolone. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 22/10/20 alle ore 09:06:26