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Titolo:
HL-60 cells degrade alpha-actinin to produce a fragment that promotes monocyte/macrophage maturation
Autore:
Masri, M; Wahl, D; Oegema, T; Luikart, S;
Indirizzi:
VA Med Ctr, Hematol Oncol 111E, Minneapolis, MN 55417 USA VA Med Ctr Minneapolis MN USA 55417 Oncol 111E, Minneapolis, MN 55417 USA Univ Minnesota, Dept Med, Minneapolis, MN USA Univ Minnesota Minneapolis MN USA nnesota, Dept Med, Minneapolis, MN USA Univ Minnesota, Dept Biochem & Orthoped Surg, Minneapolis, MN USA Univ Minnesota Minneapolis MN USA m & Orthoped Surg, Minneapolis, MN USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 2, volume: 27, anno: 1999,
pagine: 345 - 352
SICI:
0301-472X(199902)27:2<345:HCDATP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTRATE-ATTACHED MATERIAL; VIRUS-TRANSFORMED CELLS; EXTRACELLULAR-MATRIX; FIBRONECTIN FRAGMENTS; BINDING PROTEINS; HUMAN-MONOCYTES; LEUKEMIA-CELLS; DIFFERENTIATION; APOPTOSIS; MACROPHAGES;
Keywords:
actinin; extracellular matrix; monocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Luikart, S VA Med Ctr, Hematol Oncol 111E, 1 Vet Dr, Minneapolis, MN 55417USA VA Med Ctr 1 Vet Dr Minneapolis MN USA 55417 olis, MN 55417 USA
Citazione:
M. Masri et al., "HL-60 cells degrade alpha-actinin to produce a fragment that promotes monocyte/macrophage maturation", EXP HEMATOL, 27(2), 1999, pp. 345-352

Abstract

An amino-terminal fragment of oc-actinin can promote monocyte/macrophage maturation. This fragment was initially isolated from media of HL-60 myeloidleukemia cells cultured on extracellular bone marrow matrix. To determine the source of this fragment in this culture system, we investigated whetherHL-60 cells grown on bone marrow stroma have increased intracellular levels of alpha-actinin that may be released into the media during cell apoptosis, HL-60 cells grown on matrix showed no evidence of increased cellular alpha-actinin compared to cells grown on plastic substrata as measured by flowcytometry, In addition, there was no evidence of increased apoptosis as determined by DNA fragmentation assays or flow cytometry, However, 100 kD alpha-actinin was found in the extracellular matrix of bone marrow stroma by Western blot analysis and immunofluorescence microscopy, The ol-actinin content in the stroma was markedly decreased after exposure to HL-60 cells. Furthermore, lysates of HL-60 cells or of peripheral blood monocytes can degrade exogenous alpha-actinin to produce a 31 kD fragment, which promotes monocyte/macrophage maturation. We conclude that when alpha-actinin is present in the extracellular matrix, it can be modified by HL-60 cells to produce amaturation promoting 31 kD fragment. (C) 1999 International Society for Experimental Hematology. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:38:01