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Titolo:
Horseradish peroxidase immobilized electrode for phenothiazine analysis
Autore:
Petit, C; Murakami, K; Erdem, A; Kilinc, E; Borondo, GO; Liegeois, JF; Kauffmann, JM;
Indirizzi:
Free Univ Brussels, Inst Pharmaceut, B-1050 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1050 eut, B-1050 Brussels, Belgium Tokyo Kasei Univ, Lab Environm & Organ Chem, Itabashi Ku, Tokyo 173, JapanTokyo Kasei Univ Tokyo Japan 173 gan Chem, Itabashi Ku, Tokyo 173, Japan Ege Univ, Fac Pharm, Izmir, Turkey Ege Univ Izmir TurkeyEge Univ, Fac Pharm, Izmir, Turkey Univ Complutense Madrid, Dept Chem, E-28040 Madrid, Spain Univ ComplutenseMadrid Madrid Spain E-28040 Chem, E-28040 Madrid, Spain Univ Liege, Med Chem Lab, B-4000 Liege, Belgium Univ Liege Liege BelgiumB-4000 ege, Med Chem Lab, B-4000 Liege, Belgium
Titolo Testata:
ELECTROANALYSIS
fascicolo: 18, volume: 10, anno: 1998,
pagine: 1241 - 1248
SICI:
1040-0397(199812)10:18<1241:HPIEFP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROGEN-PEROXIDE; ENZYMATIC OXIDATION; CHLORPROMAZINE; INACTIVATION; FUNDAMENTALS; GENERATION; PHENOL; PHASE; DRUGS; ASSAY;
Keywords:
horseradish peroxidase; biosensor; phenothiazine; peroxidation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kauffmann, JM Frees,niv Brussels, Inst Pharmaceut, Campus Plaine CP 205-6,B-1050 Brussel Free Univ Brussels Campus Plaine CP 205-6 Brussels BelgiumB-1050
Citazione:
C. Petit et al., "Horseradish peroxidase immobilized electrode for phenothiazine analysis", ELECTROANAL, 10(18), 1998, pp. 1241-1248

Abstract

A horseradish peroxidase (HRP) immobilized carbon composite electrode has been developed for the amperometric study of phenothiazine analogues. Flow injection analysis and batch experiments have been realized in acetate buffer in the presence of hydrogen peroxide. Cyclic voltammetry and amperometryusing a thin-layer Bow cell (dual configuration, serial mode) have permitted one to suggest the mechanisms governing the biosensor signal at -0.1 V (vs. Ag/Ag+), in the presence of hydrogen peroxide, by addition of a phenothiazine derivative. It was inferred that electron transfer mediation by the phenothiazine occcurred at HRP/graphite adsorbed sites, in addition to peroxidation by dispersed HRP with substrate recycling at the graphite array-like structure of the biosensor. Thanks to these processes, high sensitivities were achieved especially in batch configurations, with amperometric detection capabilities down to 10(-8) M in acetate buffer pH 4.7. Application ofthe biosensor to the determination of phenothiazines in drug formulations were realized.

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Documento generato il 29/11/20 alle ore 00:53:02