Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Injection of sperm extract mimics spatiotemporal dynamics of Ca2+ responses and progression of meiosis at fertilization of ascidian oocytes
Autore:
Kyozuka, K; Deguchi, R; Mohri, T; Miyazaki, S;
Indirizzi:
Tohoku Univ, Asamushi Marine Biol Stn, Asamushi, Aomori 0393501, Japan Tohoku Univ Asamushi Aomori Japan 0393501 Asamushi, Aomori 0393501, Japan Natl,Inst Physiol Sci, Dept Mol Physiol, Lab Intracellular Metabol, Okazaki Natl Inst Physiol Sci Okazaki Aichi Japan 4448585 llular Metabol, Okazaki Tokyoapanens Med Univ, Sch Med, Dept Physiol, Shinjuku Ku, Tokyo 1628666, J Tokyo Womens Med Univ Tokyo Japan 1628666 Shinjuku Ku, Tokyo 1628666, J
Titolo Testata:
DEVELOPMENT
fascicolo: 20, volume: 125, anno: 1998,
pagine: 4099 - 4105
SICI:
0950-1991(199810)125:20<4099:IOSEMS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EGGS; CALCIUM OSCILLATIONS; INTRACELLULAR CA2+; MEIOTIC MATURATION; MAMMALIAN EGGS; MOUSE OOCYTES; ACTIVATION; TRIGGERS; WAVES; MESSENGERS;
Keywords:
fertilization; ascidian oocyte; sperm factor; meiotic division; intracellular calcium; calcium wave; calcium oscillation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kyozuka, K Tohoku Univ, Asamushi Marine Biol Stn, Asamushi, Aomori 0393501, Japan Tohoku Univ Asamushi Aomori Japan 0393501 omori 0393501, Japan
Citazione:
K. Kyozuka et al., "Injection of sperm extract mimics spatiotemporal dynamics of Ca2+ responses and progression of meiosis at fertilization of ascidian oocytes", DEVELOPMENT, 125(20), 1998, pp. 4099-4105

Abstract

Sperm extract (SE) of the ascidian, Ciona savignyi, injected into oocytes induced repetitive intracellular Ca2+ increases with kinetics consistent with those at fertilization and caused reinitiation and progression of meiosis as in fertilized oocytes with the formation of polar bodies. The Ca2+ response comprised two sets of Ca2+ oscillations separated by 5 minutes and correlated with the first and second meiotic metaphase, The effects of SE were dose dependent and the critical dose corresponded roughly to a single spermatozoon. In the first Ca2+ transient observed by confocal microscopy, a Ca2+ wave started from the SE injection site at the peripheral region of theoocyte and propagated across the ooplasm, The similar wave was produced byinjection at the central region, starting from an arbitrary cortical area after 30 seconds, probably after SE had diffused to the cortex. The sensitivity to SE is thought to be preferentially higher in the cortex. The effective component of SE was heat-unstable, and its molecular weight was estimated as in the range between 10x10(4) and 3x10(4) using membrane filters. These results suggest that, in ascidian fertilization, a cytosolic sperm protein factor is introduced to the oocyte cortex and induces Ca2+ waves and thereby meiotic resumption, leading to cell-cycle-correlated Ca2+ oscillations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:14:19