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Titolo:
Immunohistochemical analysis of cyclic AMP response element binding protein phosphorylation in focal cerebral ischemia in rats
Autore:
Tanaka, K; Nagata, E; Suzuki, S; Dembo, T; Nogawa, S; Fukuuchi, Y;
Indirizzi:
Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 Neurol, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 818, anno: 1999,
pagine: 520 - 526
SICI:
0006-8993(19990213)818:2<520:IAOCAR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-FOS; ARTERY OCCLUSION; MESSENGER-RNA; CREB PHOSPHORYLATION; TRANSCRIPTION; EXPRESSION; INDUCTION; NUCLEUS; BRAIN; JUN;
Keywords:
cerebral ischemia; CREB; phosphorylation; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, K Keio2,niv, Sch Med, Dept Neurol, Shinjuku Ku, 35 Shinanomachi, Tokyo 160858 Keio Univ 35 Shinanomachi Tokyo Japan 1608582 chi, Tokyo 160858
Citazione:
K. Tanaka et al., "Immunohistochemical analysis of cyclic AMP response element binding protein phosphorylation in focal cerebral ischemia in rats", BRAIN RES, 818(2), 1999, pp. 520-526

Abstract

Phosphorylation of cyclic AMP response element binding protein (CREB) is one of the most important mechanisms controlling various gene transcriptions. In the present study, the phosphorylation of CREB was examined immunohistochemically at 24 h of recirculation following 1.5 h of middle cerebral artery occlusion (MCAO) in rats. MCAO was induced by the intraluminal suture method. The infarct core revealed a significant reduction in the number of immunoreactive cells with the anti-phosphorylated CREB and with the anti-CREB antibody, which binds to both unphosphorylated and phosphorylated CREB. In contrast, the peri-infarct area exhibited a marked increase in the numberof immunopositive cells as well as in the intensity of nuclear staining with each antibody, so that almost all of the cells expressing CREB demonstrated phosphorylation of CREB. On the other hand, about half of the CREB immunopositive cells reacted weakly with the anti-phosphorylated CREB antibody in the sham group. These findings indicated that the expression as well as phosphorylation of CREB protein was significantly activated in the regions surrounding the infarct area. Since phosphorylation of CREB has recently been implicated in signal transductions that promote the survival and differentiation of neurons, the present data suggest that tissue repair mechanismsmay be markedly activated in the peri-infarct area. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:25:46